PMID- 28169322
OWN - NLM
STAT- MEDLINE
DCOM- 20181030
LR  - 20240324
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Feb 7
TI  - Collagen scaffold microenvironments modulate cell lineage commitment for 
      differentiation of bone marrow cells into regulatory dendritic cells.
PG  - 42049
LID - 10.1038/srep42049 [doi]
LID - 42049
AB  - The microenvironment plays a pivotal role for cell survival and functional 
      regulation, and directs the cell fate determination. The biological functions of 
      DCs have been extensively investigated to date. However, the influences of the 
      microenvironment on the differentiation of bone marrow cells (BMCs) into 
      dendritic cells (DCs) are not well defined. Here, we established a 3D collagen 
      scaffold microenvironment to investigate whether such 3D collagen scaffolds could 
      provide a favourable niche for BMCs to differentiate into specialised DCs. We 
      found that BMCs embedded in the 3D collagen scaffold differentiated into a 
      distinct subset of DC, exhibiting high expression of CD11b and low expression of 
      CD11c, co-stimulator (CD40, CD80, CD83, and CD86) and MHC-II molecules compared 
      to those grown in 2D culture. DCs cultured in the 3D collagen scaffold possessed 
      weak antigen uptake ability and inhibited T-cell proliferation in vitro; in 
      addition, they exhibited potent immunoregulatory function to alleviate allo-delay 
      type hypersensitivity when transferred in vivo. Thus, DCs differentiated in the 
      3D collagen scaffold were defined as regulatory DCs, indicating that collagen 
      scaffold microenvironments probably play an important role in modulating the 
      lineage commitment of DCs and therefore might be applied as a promising tool for 
      generation of specialised DCs.
FAU - Fang, Yongxiang
AU  - Fang Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
FAU - Wang, Bin
AU  - Wang B
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Zhao, Yannan
AU  - Zhao Y
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Li, Jing
AU  - Li J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Cui, Yi
AU  - Cui Y
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
AD  - Reproductive and Genetic Center of National Research Institute for Family 
      Planning, Beijing 100191, China.
FAU - Han, Sufang
AU  - Han S
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Wei, Jianshu
AU  - Wei J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Chen, Bing
AU  - Chen B
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Han, Jin
AU  - Han J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Meng, Qingyuan
AU  - Meng Q
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Hou, Xianglin
AU  - Hou X
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Luo, Jianxun
AU  - Luo J
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
FAU - Dai, Jianwu
AU  - Dai J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100190, China.
FAU - Jing, Zhizhong
AU  - Jing Z
AD  - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of 
      Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170207
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (Collagen Type I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/immunology
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/cytology/drug effects/*immunology
MH  - CD11b Antigen/genetics/immunology
MH  - CD11c Antigen/genetics/immunology
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Coculture Techniques
MH  - Collagen Type I/isolation & purification/*pharmacology
MH  - Dendritic Cells/cytology/drug effects/*immunology/transplantation
MH  - Gene Expression
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Hypersensitivity, Delayed/immunology/pathology/*therapy
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/cytology/immunology
MH  - Tendons/chemistry
MH  - *Tissue Scaffolds
PMC - PMC5294561
COIS- The authors declare no competing financial interests.
EDAT- 2017/02/09 06:00
MHDA- 2018/10/31 06:00
PMCR- 2017/02/07
CRDT- 2017/02/08 06:00
PHST- 2016/07/19 00:00 [received]
PHST- 2017/01/05 00:00 [accepted]
PHST- 2017/02/08 06:00 [entrez]
PHST- 2017/02/09 06:00 [pubmed]
PHST- 2018/10/31 06:00 [medline]
PHST- 2017/02/07 00:00 [pmc-release]
AID - srep42049 [pii]
AID - 10.1038/srep42049 [doi]
PST - epublish
SO  - Sci Rep. 2017 Feb 7;7:42049. doi: 10.1038/srep42049.