PMID- 2817426
OWN - NLM
STAT- MEDLINE
DCOM- 19891220
LR  - 20181130
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 225
IP  - 2
DP  - 1989 Oct
TI  - Effects of cholecystokinin and carbamylcholine on Paneth cell secretion in mice: 
      a comparison with pancreatic acinar cells.
PG  - 124-32
AB  - To confirm whether the Paneth cells of mice (ICR, male, 10-12 weeks old) have the 
      same secretory response to hormonal and cholinergic stimulation as do pancreatic 
      acinar cells, ultrastructural changes of Paneth cells and pancreatic acinar cells 
      1 hr after administration of various doses of cholecystokinin (octapeptide, 
      CCK-8) and carbamylcholine were morphometrically assessed. After maximal (1.5 
      micrograms/kg intraperitoneally [i.p.]) and supramaximal (15 micrograms/kg, i.p.) 
      stimulation by CCK-8, pancreatic acinar cells showed, respectively, degranulation 
      or disturbance of secretion (e.g., an increase in lysosome-like bodies, 
      aggregation of zymogen granules). The Paneth cells, however, were almost 
      unchanged in the parameters examined. After carbamylcholine injection (1,000 
      micrograms/kg, subcutaneously [s.c.]), both pancreatic acinar cells and Paneth 
      cells showed degranulation. Paneth cells sometimes developed large vacuoles, 
      probably formed after massive exocytosis; such vacuoles were not observed in 
      pancreatic acinar cells. It is suggested that Paneth cells and pancreatic acinar 
      cells have different secretory responses. Paneth cell secretion, which possibly 
      plays a role in controlling the intestinal bacterial milieu, may be stimulated by 
      cholinergic rather than hormonal mechanisms.
FAU - Satoh, Y
AU  - Satoh Y
AD  - Department of Anatomy, Asahikawa Medical College, Japan.
FAU - Ishikawa, K
AU  - Ishikawa K
FAU - Oomori, Y
AU  - Oomori Y
FAU - Yamano, M
AU  - Yamano M
FAU - Ono, K
AU  - Ono K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 8Y164V895Y (Carbachol)
RN  - 9011-97-6 (Cholecystokinin)
SB  - IM
MH  - Animals
MH  - Carbachol/*pharmacology
MH  - Cholecystokinin/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Intestinal Mucosa/*cytology/metabolism/ultrastructure
MH  - Intestine, Small/*cytology/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron
MH  - Pancreas/cytology/metabolism/ultrastructure
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
AID - 10.1002/ar.1092250207 [doi]
PST - ppublish
SO  - Anat Rec. 1989 Oct;225(2):124-32. doi: 10.1002/ar.1092250207.