PMID- 28174669
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2155-1790 (Print)
IS  - 2155-1790 (Electronic)
IS  - 2155-1790 (Linking)
VI  - 9
IP  - 1-2
DP  - 2017 Jan 8
TI  - Cell Therapy for Liver Disease Using Bioimaging Rats.
PG  - 3-7
LID - 10.3727/215517916X693104 [doi]
AB  - Advances in stem cell research suggest that cell therapy is a potential 
      alternative to liver transplantation. The use of individualized and minimally 
      invasive cell therapy is desirable to avoid rejection and reduce patient burden. 
      While allo-hepatocyte transplantation has been performed for metabolic hepatic 
      disease, auto-bone marrow transplantation (BMT) has shifted toward mesenchymal 
      stem cells (MSCs) transplantation for liver cirrhosis. In this article, an 
      overview of cell transplantation research for liver disease is provided through 
      our recent rat studies. We have developed various kinds of rat imaging models and 
      have evaluated the effect of cell therapy for liver disease. Bone marrow cells 
      (BMCs) of the Alb-DsRed2 rat were transplanted via the portal vein (PV) in acute 
      and chronic liver damage models. The number of Alb-DsRed2(+) albumin-producing 
      cells increased, and the size of the cells increased in the chronic liver damage 
      model as well as in the acute liver damage model. Luciferase transgenic (luc-Tg) 
      rat hepatocytes were transplanted into the hepatectomized LEW rat via the PV. 
      Luminescence intensity lasted for 2 months in the hepatectomized rat. BMCs 
      obtained from green fluorescent protein (GFP) Tg rats were transplanted 
      repeatedly via the PV using an implanted catheter with a port. Repeated BMT via 
      the PV reduced the liver fibrosis. Adipocyte-derived MSCs from the luc-Tg rat 
      were transplanted into the hepatectomized rat model via the PV after ischemic 
      reperfusion. MSCs inhibited hepatocyte apoptosis and promoted liver regeneration. 
      Transplanting the optimal number of cells by an effective and safe way is 
      important for clinical application. Bioimaging rats are a powerful tool for cell 
      transplantation research because it makes observation of the in vivo kinetics of 
      transplanted cells possible. Cell transplantation research using bioimaging rats 
      contributes greatly to evaluating effective methods of cell therapy.
FAU - Haga, Junko
AU  - Haga J
AD  - Department of Organ Fabrication, Keio University School of Medicine , Tokyo , 
      Japan.
FAU - Enosawa, Shin
AU  - Enosawa S
AD  - Department of Organ Fabrication, Keio University School of Medicine , Tokyo , 
      Japan.
FAU - Kobayashi, Eiji
AU  - Kobayashi E
AD  - Department of Organ Fabrication, Keio University School of Medicine , Tokyo , 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20161021
PL  - United States
TA  - Cell Med
JT  - Cell medicine
JID - 101544564
PMC - PMC5225672
OTO - NOTNLM
OT  - Bioimaging rat
OT  - Bone marrow cells (BMCs)
OT  - Cell transplantation
OT  - Liver
OT  - Mesenchymal stem cells (MSCs)
EDAT- 2017/02/09 06:00
MHDA- 2017/02/09 06:01
PMCR- 2016/10/21
CRDT- 2017/02/09 06:00
PHST- 2017/02/09 06:00 [entrez]
PHST- 2017/02/09 06:00 [pubmed]
PHST- 2017/02/09 06:01 [medline]
PHST- 2016/10/21 00:00 [pmc-release]
AID - CM112 [pii]
AID - 10.3727/215517916X693104 [doi]
PST - epublish
SO  - Cell Med. 2016 Oct 21;9(1-2):3-7. doi: 10.3727/215517916X693104. eCollection 2017 
      Jan 8.