PMID- 28176351
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20181022
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 37
IP  - 8
DP  - 2017 Aug
TI  - Role of connexin 43 in cadmium-induced proliferation of human prostate epithelial 
      cells.
PG  - 933-942
LID - 10.1002/jat.3441 [doi]
AB  - Connexins (Cxs), the subunits of gap junction channels, are involved in many 
      physiological processes. Aberrant control of Cxs and gap junction intercellular 
      communication may contribute to many diseases, including the promotion of cancer. 
      Cd exposure is associated with increased risk of human prostate cancer and benign 
      prostatic hyperplasia. The roles of Cxs in the effects of Cd on the prostate 
      have, however, not been reported previously. In this study, the human prostate 
      epithelial cell line RWPE-1 was exposed to Cd. A low dose of Cd stimulated cell 
      proliferation along with a lower degree of gap junction intercellular 
      communication and an elevated level of the protein Cx43. Cd exposure increased 
      the levels of intracellular Ca(2+) and phosphorylated Cx43 at the Ser368 site. 
      Knockdown of Cx43 using siRNA blocked Cd-induced proliferation and interfered 
      with the Cd-induced changes in the protein levels of cyclin D1, cyclin B1, 
      p27(Kip1) (p27) and p21(Waf1/Cip1) (p21). The increase in Cx43 expression induced 
      by Cd was presumably mediated by the androgen receptor, because it was abolished 
      upon treatment with the androgen receptor antagonist, flutamide. Thus, a low dose 
      of Cd promotes cell proliferation in RWPE-1, possibly mediated by Cx43 expression 
      through an effect on cell cycle-associated proteins. Cx43 might be a target for 
      prostatic diseases associated with Cd exposure. Copyright (c) 2017 John Wiley & 
      Sons, Ltd.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Liu, Qingping
AU  - Liu Q
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
FAU - Ji, Xiaoli
AU  - Ji X
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
FAU - Ge, Zehe
AU  - Ge Z
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
FAU - Diao, Haipeng
AU  - Diao H
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
FAU - Chang, Xiuli
AU  - Chang X
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
FAU - Wang, Lihua
AU  - Wang L
AD  - Shanghai Jinshan District Center for Disease Control & Prevention, Weisheng Road, 
      Jinshan District, Shanghai, 201599, China.
FAU - Wu, Qing
AU  - Wu Q
AUID- ORCID: 0000-0002-6124-2594
AD  - School of Public Health, Fudan University, DongAn Road, Shanghai, 200032, China.
AD  - Key Laboratory of Public Health Safety, Ministry of Education, DongAn Road, 
      Shanghai, 200032, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170208
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Connexin 43)
RN  - 0 (Environmental Pollutants)
RN  - 0 (GJA1 protein, human)
RN  - 00BH33GNGH (Cadmium)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cadmium/*toxicity
MH  - Cell Communication/drug effects
MH  - Cell Culture Techniques
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Connexin 43/genetics/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Environmental Pollutants/*toxicity
MH  - Epithelial Cells/*drug effects/metabolism/pathology
MH  - Gap Junctions/drug effects/metabolism
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - Prostate/*drug effects/metabolism/pathology
OTO - NOTNLM
OT  - Cx43
OT  - GJIC
OT  - androgen receptor
OT  - cadmium
OT  - cell proliferation
OT  - prostate
EDAT- 2017/02/09 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/02/09 06:00
PHST- 2016/10/25 00:00 [received]
PHST- 2016/12/21 00:00 [revised]
PHST- 2016/12/23 00:00 [accepted]
PHST- 2017/02/09 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/02/09 06:00 [entrez]
AID - 10.1002/jat.3441 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2017 Aug;37(8):933-942. doi: 10.1002/jat.3441. Epub 2017 Feb 8.