PMID- 28179296
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20211204
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 2
DP  - 2017 Feb
TI  - Itraconazole Inhibits AKT/mTOR Signaling and Proliferation in Endometrial Cancer 
      Cells.
PG  - 515-519
AB  - BACKGROUND: Itraconazole is a common antifungal agent that has demonstrated 
      anticancer activity in preclinical and clinical studies. This study investigated 
      whether itraconazole exerts this effect in endometrial cancer (EC) cells. 
      MATERIALS AND METHODS: Cell viability was evaluated with the 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and gene and 
      protein expression were assessed by microarray analysis and immunoblotting, 
      respectively, in five EC cell lines. RESULTS: Itraconazole-suppressed 
      proliferation of AN3-CA, HEC-1A and Ishikawa cells (p<0.05) but not of HEC-50B or 
      SNG-II cells. Itraconazole did not suppress GLI1 or GLI2 transcription but did 
      inhibit the expression of mammalian target of rapamycin (mTOR) signaling 
      components in AN3-CA and HEC-1A cells, while inducing that of 
      microtubule-associated protein 1A/1B-light chain 3-II, a marker of autophagy. 
      ATP-binding cassette transporter A1 gene was down-regulated in Ishikawa, HEC-50B 
      and SNG-II cells. CONCLUSION: Itraconazole treatment suppresses the growth of EC 
      cells by inhibiting AKT/mTOR signalling.
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Tsubamoto, Hiroshi
AU  - Tsubamoto H
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan tsuba@hyo-med.ac.jp.
AD  - Department of Medical Oncology, Meiwa Hospital, Nishinomiya, Japan.
FAU - Inoue, Kayo
AU  - Inoue K
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan.
FAU - Sakata, Kazuko
AU  - Sakata K
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan.
FAU - Ueda, Tomoko
AU  - Ueda T
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan.
FAU - Takeyama, Ryu
AU  - Takeyama R
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan.
FAU - Shibahara, Hiroaki
AU  - Shibahara H
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, 
      Japan.
FAU - Sonoda, Takashi
AU  - Sonoda T
AD  - Department of Medical Oncology, Meiwa Hospital, Nishinomiya, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 304NUG5GF4 (Itraconazole)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endometrial Neoplasms/*drug therapy/genetics/metabolism/pathology
MH  - Female
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Itraconazole/*pharmacology
MH  - Oncogene Protein v-akt/*antagonists & inhibitors/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*antagonists & inhibitors/metabolism
OTO - NOTNLM
OT  - AKT/mTOR
OT  - Itraconazole
OT  - autophagy
OT  - endometrial cancer
OT  - hedgehog
EDAT- 2017/02/10 06:00
MHDA- 2017/02/14 06:00
CRDT- 2017/02/10 06:00
PHST- 2016/11/12 00:00 [received]
PHST- 2016/12/20 00:00 [revised]
PHST- 2016/12/22 00:00 [accepted]
PHST- 2017/02/10 06:00 [entrez]
PHST- 2017/02/10 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
AID - 37/2/515 [pii]
AID - 10.21873/anticanres.11343 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 Feb;37(2):515-519. doi: 10.21873/anticanres.11343.