PMID- 28179391
OWN - NLM
STAT- MEDLINE
DCOM- 20180710
LR  - 20181113
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 7
IP  - 4
DP  - 2017 Apr 3
TI  - The Nuclear Cap-Binding Complex Mediates Meiotic Silencing by Unpaired DNA.
PG  - 1149-1155
LID - 10.1534/g3.116.038679 [doi]
AB  - In the filamentous fungus Neurospora crassa, cross walls between individual cells 
      are normally incomplete, making the entire fungal network vulnerable to attack by 
      viruses and selfish DNAs. Accordingly, several genome surveillance mechanisms are 
      maintained to help the fungus combat these repetitive elements. One of these 
      defense mechanisms is called meiotic silencing by unpaired DNA (MSUD), which 
      identifies and silences unpaired genes during meiosis. Utilizing common RNA 
      interference (RNAi) proteins, such as Dicer and Argonaute, MSUD targets mRNAs 
      homologous to the unpaired sequence to achieve silencing. In this study, we have 
      identified an additional silencing component, namely the cap-binding complex 
      (CBC). Made up of cap-binding proteins CBP20 and CBP80, CBC associates with the 
      5' cap of mRNA transcripts in eukaryotes. The loss of CBC leads to a deficiency 
      in MSUD activity, suggesting its role in mediating silencing. As confirmed in 
      this study, CBC is predominantly nuclear, although it is known to travel in and 
      out of the nucleus to facilitate RNA transport. As seen in animals but not in 
      plants, CBP20's robust nuclear import depends on CBP80 in Neurospora CBC 
      interacts with a component (Argonaute) of the perinuclear meiotic silencing 
      complex (MSC), directly linking the two cellular factors.
CI  - Copyright (c) 2017 Decker et al.
FAU - Decker, Logan M
AU  - Decker LM
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Xiao, Hua
AU  - Xiao H
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Boone, Erin C
AU  - Boone EC
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Vierling, Michael M
AU  - Vierling MM
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Shanker, Benjamin S
AU  - Shanker BS
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Kingston, Shanika L
AU  - Kingston SL
AD  - Department of Biology, Barry University, Miami Shores, Florida 33161.
FAU - Boone, Shannon F
AU  - Boone SF
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Haynes, Jackson B
AU  - Haynes JB
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211.
FAU - Shiu, Patrick K T
AU  - Shiu PK
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      shiup@missouri.edu.
LA  - eng
GR  - P01 GM068087/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170403
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - 0 (DNA, Fungal)
RN  - 0 (Nuclear Cap-Binding Protein Complex)
SB  - IM
MH  - Cell Nucleus/metabolism
MH  - DNA, Fungal/genetics/*metabolism
MH  - *Gene Silencing
MH  - Genes, Fungal
MH  - *Meiosis
MH  - Neurospora/*cytology/*genetics
MH  - Nuclear Cap-Binding Protein Complex/*metabolism
MH  - Protein Binding
PMC - PMC5386863
OTO - NOTNLM
OT  - Neurospora crassa
OT  - RNA interference (RNAi)
OT  - cap-binding proteins (CBPs)
OT  - meiosis
OT  - meiotic silencing by unpaired DNA (MSUD)
EDAT- 2017/02/10 06:00
MHDA- 2018/07/11 06:00
PMCR- 2017/02/06
CRDT- 2017/02/10 06:00
PHST- 2017/02/10 06:00 [pubmed]
PHST- 2018/07/11 06:00 [medline]
PHST- 2017/02/10 06:00 [entrez]
PHST- 2017/02/06 00:00 [pmc-release]
AID - g3.116.038679 [pii]
AID - GGG_038679 [pii]
AID - 10.1534/g3.116.038679 [doi]
PST - epublish
SO  - G3 (Bethesda). 2017 Apr 3;7(4):1149-1155. doi: 10.1534/g3.116.038679.