PMID- 28182765
OWN - NLM
STAT- MEDLINE
DCOM- 20170907
LR  - 20190208
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 2
DP  - 2017
TI  - Rapamycin/sodium hyaluronate binding on nano-hydroxyapatite coated titanium 
      surface improves MC3T3-E1 osteogenesis.
PG  - e0171693
LID - 10.1371/journal.pone.0171693 [doi]
LID - e0171693
AB  - Endosseous titanium (Ti) implant failure due to poor biocompatibility of implant 
      surface remains a major problem for osseointegration. Improving the topography of 
      Ti surface may enhance osseointegration, however, the mechanism remains unknown. 
      To investigate the effect of modified Ti surface on osteogenesis, we loaded 
      rapamycin (RA) onto nano-hydroxyapatite (HAp) coated Ti surface which was 
      acid-etched, alkali-heated and HAp coated sequentially. Sodium hyaluronate (SH) 
      was employed as an intermediate layer for the load of RA, and a steady release 
      rate of RA was maintained. Cell vitality of MC3T3-E1 was assessed by MTT. 
      Osteogenesis of MC3T3-E1 on this modified Ti surface was evaluated by alkaline 
      phosphatase (ALP) activity, mineralization and related osteogenesis genes 
      osteocalcin (OCN), osteopontin (OPN), Collagen-I and Runx2. The result revealed 
      that RA/SH-loaded nano-HAp Ti surface was innocent for cell vitality and even 
      more beneficial for cell osteogenesis in vitro. Furthermore, osteogenesis of 
      MC3T3-E1 showed significant association with the mammalian target of rapamycin 
      (mTOR) phosphorylation by RA, which required further study about the mechanism. 
      The approach to this modified Ti surface presented in this paper has high 
      research value for the development of Ti-based implant.
FAU - Liu, Chao
AU  - Liu C
AD  - Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, 
      Qilu Hospital, Shandong University, Jinan, China.
FAU - Dong, Jian Yong
AU  - Dong JY
AD  - School of Stomotology, Shandong University, Jinan, China.
FAU - Yue, Lin Lin
AU  - Yue LL
AD  - School of Stomotology, Shandong University, Jinan, China.
FAU - Liu, Shao Hua
AU  - Liu SH
AD  - Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, 
      Qilu Hospital, Shandong University, Jinan, China.
FAU - Wan, Yi
AU  - Wan Y
AD  - Key Laboratory of High Efficiency and Clean Mechanical Manufacture Ministry of 
      Education, School of Mechanical Engineering, Shandong University, Jinan, China.
FAU - Liu, Hong
AU  - Liu H
AD  - State Key Laboratory of Crystal Materials, Center of Bio & Micro/nano Functional 
      Materials, Shandong University, Jinan, PR China.
FAU - Tan, Wan Ye
AU  - Tan WY
AD  - Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, 
      Qilu Hospital, Shandong University, Jinan, China.
FAU - Guo, Qian Qian
AU  - Guo QQ
AD  - School of Stomotology, Shandong University, Jinan, China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Department of Oral and Maxillofacial Surgery and Institute of Dental Medicine, 
      Qilu Hospital, Shandong University, Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20170209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Coated Materials, Biocompatible)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 91D9GV0Z28 (Durapatite)
RN  - D1JT611TNE (Titanium)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coated Materials, Biocompatible/chemistry/*pharmacology
MH  - Durapatite/chemistry/*pharmacology
MH  - Hyaluronic Acid/chemistry/pharmacology
MH  - Mice
MH  - Nanostructures/chemistry
MH  - Osseointegration/drug effects
MH  - Osteoblasts/drug effects/physiology
MH  - Osteogenesis/*drug effects
MH  - Prostheses and Implants
MH  - Sirolimus/chemistry/*pharmacology
MH  - Surface Properties
MH  - Tissue Scaffolds/chemistry
MH  - Titanium/chemistry/*pharmacology
PMC - PMC5300161
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/02/10 06:00
MHDA- 2017/09/08 06:00
PMCR- 2017/02/09
CRDT- 2017/02/10 06:00
PHST- 2016/08/05 00:00 [received]
PHST- 2017/01/23 00:00 [accepted]
PHST- 2017/02/10 06:00 [entrez]
PHST- 2017/02/10 06:00 [pubmed]
PHST- 2017/09/08 06:00 [medline]
PHST- 2017/02/09 00:00 [pmc-release]
AID - PONE-D-16-31382 [pii]
AID - 10.1371/journal.pone.0171693 [doi]
PST - epublish
SO  - PLoS One. 2017 Feb 9;12(2):e0171693. doi: 10.1371/journal.pone.0171693. 
      eCollection 2017.