PMID- 28185819
OWN - NLM
STAT- MEDLINE
DCOM- 20170315
LR  - 20170315
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 173
DP  - 2017 Mar 15
TI  - Expression of polysialic acid in primary laryngeal squamous cell carcinoma.
PG  - 73-79
LID - S0024-3205(17)30039-5 [pii]
LID - 10.1016/j.lfs.2017.02.002 [doi]
AB  - AIMS: Expression of polySia is associated with metastatic dissemination and 
      progression of various malignant diseases. In particular, it may contribute to 
      tumorigenesis by a negative modulatory effect on cellular signaling cascades 
      responsible for cellular migration, differentiation and proliferation. In this 
      study, we investigated the expression of polySia in primary metastatic and 
      non-metastatic laryngeal squamous cell carcinoma (LSCC) tumor tissues and its 
      potential impact on the LSCC progression. MAIN METHODS: The expression of polySia 
      in metastatic and non-metastatic primary laryngeal squamous cell carcinoma (LSCC) 
      tumor biopsy specimens was investigated by immunohistochemistry, while the 
      expression of polysialyltransferase IV (ST8SiaIV)(), fibroblast growth factor 
      receptor 1 (FGFR1), extracellular signal regulated kinases 1 and 2 (Erk 1/2) and 
      c-Raf was tested in metastatic and non-metastatic primary tumor tissues 
      (including the corresponding non-tumor control tissues) by Western blot analysis. 
      KEY FINDINGS: The expression of polySia was detected in LSCC biopsies specimens 
      with generally stronger immunoreactivity in non-metastatic tumor LSCC sections 
      and in histologically undifferentiated tumors. Also, increased polySia expression 
      was observed in adjacent histologically unaltered laryngeal tumor-associated 
      tissue of the metastatic sections. In addition, we provide an evidence of 
      increased polysialyltransferase IV (ST8SiaIV) expression, involved in polySia 
      synthesis in both metastatic and non-metastatic primary tumors which is 
      accompanied by decreased levels of FGFR1, Erk 1/2 and c-Raf. SIGNIFICANCE: We 
      present for the first time the evidence for the polySia expression in LSCC 
      biopsies specimens which suggests its potential impact on initial steps of LSCC 
      malignant transformation.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Klobucar, Marko
AU  - Klobucar M
AD  - University of Rijeka, Department of Biotechnology, Centre of High-Throughput 
      Technologies, Radmile Matejcic 2, 51000 Rijeka, Croatia.
FAU - Visentin, Sarah
AU  - Visentin S
AD  - University of Rijeka, Department of Biotechnology, Centre of High-Throughput 
      Technologies, Radmile Matejcic 2, 51000 Rijeka, Croatia.
FAU - Jakovcevic, Antonija
AU  - Jakovcevic A
AD  - University Department of ENT, Head and Neck Surgery, Kispaticeva 12, 10 000 
      Zagreb, Croatia.
FAU - Bilic, Mario
AU  - Bilic M
AD  - University Department of ENT, Head and Neck Surgery, Kispaticeva 12, 10 000 
      Zagreb, Croatia.
FAU - Kovac-Bilic, Lana
AU  - Kovac-Bilic L
AD  - University Department of ENT, Head and Neck Surgery, Kispaticeva 12, 10 000 
      Zagreb, Croatia.
FAU - Danic, Davorin
AU  - Danic D
AD  - University Josip Juraj Strossmayer of Osijek, Medical School Osijek, Department 
      of ENT and Head and Neck Surgery, General Hospital "Dr. J. Bencevic", 35 000 
      Slavonski Brod, Croatia.
FAU - Pavelic, Kresimir
AU  - Pavelic K
AD  - University of Rijeka, Department of Biotechnology, Centre of High-Throughput 
      Technologies, Radmile Matejcic 2, 51000 Rijeka, Croatia.
FAU - Kraljevic Pavelic, Sandra
AU  - Kraljevic Pavelic S
AD  - University of Rijeka, Department of Biotechnology, Centre of High-Throughput 
      Technologies, Radmile Matejcic 2, 51000 Rijeka, Croatia. Electronic address: 
      sandrakp@biotech.uniri.hr.
LA  - eng
PT  - Journal Article
DEP - 20170207
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialic acid)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 3.4.99.- (ST8SIA4 protein, human)
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/biosynthesis
MH  - Sialic Acids/*biosynthesis
MH  - Sialyltransferases/biosynthesis
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - FGFR-MAPK/Erk1/2 signaling
OT  - Laryngeal squamous cell carcinoma
OT  - Metastases
OT  - Polysialic acid
OT  - Polysialyltransferase
EDAT- 2017/02/12 06:00
MHDA- 2017/03/16 06:00
CRDT- 2017/02/11 06:00
PHST- 2016/12/24 00:00 [received]
PHST- 2017/01/27 00:00 [revised]
PHST- 2017/02/02 00:00 [accepted]
PHST- 2017/02/12 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
PHST- 2017/02/11 06:00 [entrez]
AID - S0024-3205(17)30039-5 [pii]
AID - 10.1016/j.lfs.2017.02.002 [doi]
PST - ppublish
SO  - Life Sci. 2017 Mar 15;173:73-79. doi: 10.1016/j.lfs.2017.02.002. Epub 2017 Feb 7.