PMID- 28186602
OWN - NLM
STAT- MEDLINE
DCOM- 20170629
LR  - 20170629
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 34
IP  - 1
DP  - 2017 Feb 10
TI  - [Analysis of isodicentric Ph chromosomes in chronic myeloid leukemia blast 
      crisis].
PG  - 85-88
LID - 10.3760/cma.j.issn.1003-9406.2017.01.020 [doi]
AB  - OBJECTIVE: To explore the genetic and clinical characteristics of isodicentric Ph 
      chromosomes [idic(Ph)] in lymphoid blast crisis of chronic myeloid leukemia 
      (CML-BLC). METHODS: Bone marrow aspirates of 2 patients with CML-BLC were 
      analyzed by R banding after 24 hours of culturing. Genomic copy number variations 
      (CNV) were analyzed by single nucleotide polymorphism array (SNP array) in case 
      1. The results were confirmed with fluorescence in situ hybridization (FISH). 
      Variations of acute lymphoblastic leukemia-related genes including CDKN2A/AB and 
      PAX5 were detected by multiplex ligation-dependent probe amplication (MLPA). 
      RESULTS: Deletions and duplications on derivative chromosome 9 detected by FISH 
      were confirmed by SNP array analysis. The distances between the BCR/ABL fusion 
      signals on the idic(Ph) chromosomes in the two patients have differed greatly. 
      The idic(Ph) in the second patient was supposed to be formed by two Ph 
      chromosomes joined at their q terminals, where as the idic(Ph) in the first 
      patient have been shown to be fused at the satellite regions of their p arms. 
      CONCLUSION: The idic(Ph) chromosomes presented in CML-BLC may predict resistance 
      to Imatinib and response to Dasatinib.
FAU - Li, Qian
AU  - Li Q
AD  - Hematological Laboratory, Department of Laboratory Medicine, The Second 
      Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325006, China. 
      wzchenhui@126.com.
FAU - Lin, Xiaoji
AU  - Lin X
FAU - Lin, Ying
AU  - Lin Y
FAU - Yao, Rongxin
AU  - Yao R
FAU - Huang, Wu
AU  - Huang W
FAU - Mei, Handong
AU  - Mei H
FAU - Gong, Jian
AU  - Gong J
FAU - Chen, Hui
AU  - Chen H
FAU - Teng, Ningyan
AU  - Teng N
LA  - chi
PT  - Case Reports
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Blast Crisis/diagnosis/*genetics/therapy
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Deletion
MH  - Chromosome Duplication
MH  - Chromosomes, Human, Pair 9/genetics
MH  - DNA Copy Number Variations
MH  - Fatal Outcome
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/*genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
EDAT- 2017/02/12 06:00
MHDA- 2017/07/01 06:00
CRDT- 2017/02/11 06:00
PHST- 2017/02/11 06:00 [entrez]
PHST- 2017/02/12 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
AID - 940634020 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2017.01.020 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017 Feb 10;34(1):85-88. doi: 
      10.3760/cma.j.issn.1003-9406.2017.01.020.