PMID- 28187222
OWN - NLM
STAT- MEDLINE
DCOM- 20171004
LR  - 20180515
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Linking)
VI  - 102
IP  - 7
DP  - 2017 Jul 1
TI  - Impaired Release of Vitamin D in Dysfunctional Adipose Tissue: New Cues on 
      Vitamin D Supplementation in Obesity.
PG  - 2564-2574
LID - 10.1210/jc.2016-3591 [doi]
AB  - CONTEXT: Vitamin D accumulates in adipose tissue (AT), and vitamin D deficiency 
      is frequent in obesity. OBJECTIVE: We hypothesize that trafficking of vitamin D 
      is altered in dysfunctional AT. DESIGN, PATIENTS, SETTINGS: Fifty-four 
      normal-weight and 67 obese males were recruited in a prospective study and 
      randomly assigned to supplementation with 50 microg/wk 25-hydroxyvitamin-D3 or 150 
      microg/wk vitamin D3 for 1 year, raising dosage by 50% if vitamin D sufficiency 
      [serum 25-hydroxyvitamin-D3 >50 nmol/L], was not achieved at 6 months; 97 
      subjects completed the study. METHODS: Vitamin D3 and 25-hydroxyvitamin-D3 were 
      quantified by HPLC-MS in control and insulin-resistant (IR) 3T3-L1 cells and 
      subcutaneous AT (SAT) from lean and obese subjects, incubated with or without 
      adrenaline; expression of 25-hydroxylase (Cyp27a1), 1alpha-hydroxylase (Cyp27b1), and 
      vitamin D receptor (Vdr) was analyzed by real-time polymerase chain reaction. 
      RESULTS: In IR adipocytes, uptake of D3 and 25-hydroxyvitamin-D3 was higher, but, 
      after adrenaline stimulation, the decrement in D3 and 25-hydroxyvitamin-D3 was 
      stronger in control cells, which also showed increased expression of Cyp27a1 and 
      Cyp27b1 and higher levels of 25-hydroxyvitamin-D3. In SAT from obese subjects, 
      adrenaline-induced release of D3 and 25-hydroxyvitamin-D3 was blunted; in both IR 
      cells and obese SAT, protein expression of beta2-adrenergic receptor was reduced. 
      Supplementation with 25-hydroxyvitamin-D3 was more effective in achieving vitamin 
      D sufficiency in obese, but not in normal weight subjects. CONCLUSION: 
      Dysfunctional AT shows a reduced catecholamine-induced release of D3 and 
      25-hydroxyvitamin-D3 and altered activity of vitamin D-metabolizing enzymes; for 
      these reasons supplementation with 25-hydroxyvitamin-D3 is more effective in 
      obese individuals.
CI  - Copyright (c) 2017 by the Endocrine Society
FAU - Di Nisio, Andrea
AU  - Di Nisio A
AD  - Department of Medicine, Unit of Andrology and Reproductive Medicine, University 
      of Padova, 35128 Padova, Italy.
FAU - De Toni, Luca
AU  - De Toni L
AD  - Department of Medicine, Unit of Andrology and Reproductive Medicine, University 
      of Padova, 35128 Padova, Italy.
FAU - Sabovic, Iva
AU  - Sabovic I
AD  - Department of Medicine, Unit of Andrology and Reproductive Medicine, University 
      of Padova, 35128 Padova, Italy.
AD  - Familial Tumor Unit, Veneto Institute of Oncology Istituto Oncologico 
      Veneto-Istituto di Ricovero e Cura a Carattere Scientifico, 35128 Padova, Italy.
FAU - Rocca, Maria Santa
AU  - Rocca MS
AD  - Department of Medicine, Unit of Andrology and Reproductive Medicine, University 
      of Padova, 35128 Padova, Italy.
FAU - De Filippis, Vincenzo
AU  - De Filippis V
AD  - Laboratory of Protein Chemistry, Department of Pharmaceutical and Pharmacological 
      Sciences, University of Padova, 35128 Padova, Italy.
FAU - Opocher, Giuseppe
AU  - Opocher G
AD  - Familial Tumor Unit, Veneto Institute of Oncology Istituto Oncologico 
      Veneto-Istituto di Ricovero e Cura a Carattere Scientifico, 35128 Padova, Italy.
FAU - Azzena, Bruno
AU  - Azzena B
AD  - Burn Unit and Plastic Surgery, University Hospital of Padova, 35128 Padova, 
      Italy.
FAU - Vettor, Roberto
AU  - Vettor R
AD  - Department of Medicine, Internal Medicine, University of Padova, 35128 Padova, 
      Italy.
FAU - Plebani, Mario
AU  - Plebani M
AD  - Department of Medicine, Laboratory Medicine, University of Padova, 35128 Padova, 
      Italy.
FAU - Foresta, Carlo
AU  - Foresta C
AD  - Department of Medicine, Unit of Andrology and Reproductive Medicine, University 
      of Padova, 35128 Padova, Italy.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 1406-16-2 (Vitamin D)
RN  - P6YZ13C99Q (Calcifediol)
SB  - IM
MH  - Adipose Tissue/*metabolism/physiopathology
MH  - Administration, Oral
MH  - Adult
MH  - Blotting, Western
MH  - Body Mass Index
MH  - Body Weight
MH  - Calcifediol/*administration & dosage/pharmacokinetics
MH  - Case-Control Studies
MH  - *Dietary Supplements
MH  - Humans
MH  - Italy
MH  - Male
MH  - Middle Aged
MH  - Obesity/*drug therapy/metabolism
MH  - Prospective Studies
MH  - Reference Values
MH  - Treatment Outcome
MH  - Vitamin D/*administration & dosage/pharmacokinetics
EDAT- 2017/02/12 06:00
MHDA- 2017/10/05 06:00
CRDT- 2017/02/11 06:00
PHST- 2016/11/02 00:00 [received]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/02/12 06:00 [pubmed]
PHST- 2017/10/05 06:00 [medline]
PHST- 2017/02/11 06:00 [entrez]
AID - 2982841 [pii]
AID - 10.1210/jc.2016-3591 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2017 Jul 1;102(7):2564-2574. doi: 10.1210/jc.2016-3591.