PMID- 28191470
OWN - NLM
STAT- MEDLINE
DCOM- 20170615
LR  - 20190723
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2017
DP  - 2017
TI  - Effects of a Novel Glucokinase Activator, HMS5552, on Glucose Metabolism in a Rat 
      Model of Type 2 Diabetes Mellitus.
PG  - 5812607
LID - 10.1155/2017/5812607 [doi]
LID - 5812607
AB  - Glucokinase (GK) plays a critical role in the control of whole-body glucose 
      homeostasis. We investigated the possible effects of a novel glucokinase 
      activator (GKA), HMS5552, to the GK in rats with type 2 diabetes mellitus (T2DM). 
      Male Sprague-Dawley (SD) rats were divided into four groups: control group, 
      diabetic group, low-dose (10 mg/kg) HMS5552-treated diabetic group (HMS-L), and 
      high-dose (30 mg/kg) HMS5552-treated diabetic group (HMS-H). HMS5552 was 
      administered intragastrically to the T2DM rats for one month. The levels of total 
      cholesterol, triglyceride, fasting plasma insulin (FINS), and glucagon (FG) were 
      determined, and an oral glucose tolerance test was performed. The expression 
      patterns of proteins and genes associated with insulin resistance and GK activity 
      were assayed. Compared with diabetic rats, the FINS level was significantly 
      decreased in the HMS5552-treated diabetic rats. HMS5552 treatment significantly 
      lowered the blood glucose levels and improved GK activity and insulin resistance. 
      The immunohistochemistry, western blot, and semiquantitative RT-PCR results 
      further demonstrated the effects of HMS5552 on the liver and pancreas. Our data 
      suggest that the novel GKA, HMS5552, exerts antidiabetic effects on the liver and 
      pancreas by improving GK activity and insulin resistance, which holds promise as 
      a novel drug for the treatment of T2DM patients.
FAU - Wang, Ping
AU  - Wang P
AUID- ORCID: 0000-0001-9584-3809
AD  - The Key Laboratory of Pharmacology and Medical Molecular Biology, Medical 
      College, Henan University of Science and Technology, Luoyang 471023, China.
FAU - Liu, Huili
AU  - Liu H
AD  - School Clinic, Henan University of Science and Technology, Luoyang 471023, China.
FAU - Chen, Li
AU  - Chen L
AUID- ORCID: 0000-0003-0990-9790
AD  - Department of Clinical Research & Development, Hua Medicine, Shanghai 201203, 
      China.
FAU - Duan, Yingli
AU  - Duan Y
AUID- ORCID: 0000-0002-6778-8029
AD  - The Key Laboratory of Pharmacology and Medical Molecular Biology, Medical 
      College, Henan University of Science and Technology, Luoyang 471023, China.
FAU - Chen, Qunli
AU  - Chen Q
AUID- ORCID: 0000-0003-1599-168X
AD  - The Key Laboratory of Pharmacology and Medical Molecular Biology, Medical 
      College, Henan University of Science and Technology, Luoyang 471023, China.
FAU - Xi, Shoumin
AU  - Xi S
AUID- ORCID: 0000-0001-6271-4656
AD  - The Key Laboratory of Pharmacology and Medical Molecular Biology, Medical 
      College, Henan University of Science and Technology, Luoyang 471023, China.
LA  - eng
PT  - Journal Article
DEP - 20170116
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (Triglycerides)
RN  - 9007-92-5 (Glucagon)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Blood Glucose/*metabolism
MH  - Carbohydrate Metabolism/*drug effects
MH  - Cholesterol/blood
MH  - Diabetes Mellitus, Experimental/metabolism
MH  - Diabetes Mellitus, Type 2/*drug therapy/metabolism
MH  - Glucagon/blood
MH  - Glucokinase/*metabolism
MH  - Glucose Tolerance Test
MH  - Insulin/blood
MH  - Insulin Resistance
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Triglycerides/blood
PMC - PMC5278194
COIS- Li Chen is an employee of Hua Medicine, Shanghai, China. All other authors 
      declare that they have no conflict of interests.
EDAT- 2017/02/14 06:00
MHDA- 2017/06/16 06:00
PMCR- 2017/01/16
CRDT- 2017/02/14 06:00
PHST- 2016/09/29 00:00 [received]
PHST- 2016/12/01 00:00 [revised]
PHST- 2016/12/27 00:00 [accepted]
PHST- 2017/02/14 06:00 [entrez]
PHST- 2017/02/14 06:00 [pubmed]
PHST- 2017/06/16 06:00 [medline]
PHST- 2017/01/16 00:00 [pmc-release]
AID - 10.1155/2017/5812607 [doi]
PST - ppublish
SO  - J Diabetes Res. 2017;2017:5812607. doi: 10.1155/2017/5812607. Epub 2017 Jan 16.