PMID- 28193272
OWN - NLM
STAT- MEDLINE
DCOM- 20170704
LR  - 20240324
IS  - 1472-6750 (Electronic)
IS  - 1472-6750 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Feb 13
TI  - Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi 
      method of Design of experiments.
PG  - 12
LID - 10.1186/s12896-017-0333-x [doi]
LID - 12
AB  - BACKGROUND: Fungal laccase has profound applications in different fields of 
      biotechnology due to its broad specificity and high redox potential. Any 
      successful application of the enzyme requires large scale production. As laccase 
      production is highly dependent on medium components and cultural conditions, 
      optimization of the same is essential for efficient product production. RESULTS: 
      Production of laccase by fungal strain Marasmiellus palmivorus LA1 under solid 
      state fermentation was optimized by the Taguchi design of experiments (DOE) 
      methodology. An orthogonal array (L8) was designed using Qualitek-4 software to 
      study the interactions and relative influence of the seven selected factors by 
      one factor at a time approach. The optimum condition formulated was temperature 
      (28  degrees C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum 
      concentration (number of mycelial agar pieces) (6Nos.) and substrate length 
      (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. 
      Statistical optimization leads to the elimination of an insignificant medium 
      component ammonium dihydrogen phosphate from the process and contributes to a 
      1.06 fold increase in enzyme production. A final production of 667.4 +/- 13 IU/mL 
      laccase activity paves way for the application of this strain for industrial 
      applications. CONCLUSION: Study optimized lignin degrading laccases from 
      Marasmiellus palmivorus LA1. This laccases can thus be used for further 
      applications in different scales of production after analyzing the properties of 
      the enzyme. Study also confirmed the use of taguchi method for optimizations of 
      product production.
FAU - Chenthamarakshan, Aiswarya
AU  - Chenthamarakshan A
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Parambayil, Nayana
AU  - Parambayil N
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Miziriya, Nafeesathul
AU  - Miziriya N
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Soumya, P S
AU  - Soumya PS
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Lakshmi, M S Kiran
AU  - Lakshmi MS
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Ramgopal, Anala
AU  - Ramgopal A
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Dileep, Anuja
AU  - Dileep A
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India.
FAU - Nambisan, Padma
AU  - Nambisan P
AD  - Department of Biotechnology, Cochin University of Science and Technology, 
      Cochin-22, Kerala, India. plantbiotech1992@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170213
PL  - England
TA  - BMC Biotechnol
JT  - BMC biotechnology
JID - 101088663
RN  - 0 (Culture Media)
RN  - EC 1.10.3.2 (Laccase)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Basidiomycota/*cytology/*metabolism
MH  - Bioreactors/*microbiology/standards
MH  - Cell Proliferation/physiology
MH  - Computer Simulation
MH  - Culture Media/metabolism/standards
MH  - Galactose/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - India
MH  - Industrial Microbiology/methods/standards
MH  - Laccase/*biosynthesis/isolation & purification
MH  - Models, Biological
MH  - *Models, Statistical
MH  - Quality Control
MH  - Temperature
PMC - PMC5307782
OTO - NOTNLM
OT  - Laccase
OT  - Marasmiellus palmivorus LA1
OT  - Optimization
OT  - Solid state fermentation
OT  - Taguchi DOE
EDAT- 2017/02/15 06:00
MHDA- 2017/07/05 06:00
PMCR- 2017/02/13
CRDT- 2017/02/15 06:00
PHST- 2016/08/10 00:00 [received]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/02/15 06:00 [entrez]
PHST- 2017/02/15 06:00 [pubmed]
PHST- 2017/07/05 06:00 [medline]
PHST- 2017/02/13 00:00 [pmc-release]
AID - 10.1186/s12896-017-0333-x [pii]
AID - 333 [pii]
AID - 10.1186/s12896-017-0333-x [doi]
PST - epublish
SO  - BMC Biotechnol. 2017 Feb 13;17(1):12. doi: 10.1186/s12896-017-0333-x.