PMID- 28193589 OWN - NLM STAT- MEDLINE DCOM- 20170512 LR - 20181202 IS - 1879-1298 (Electronic) IS - 0045-6535 (Linking) VI - 174 DP - 2017 May TI - Head kidney, liver and skin histopathology and gene expression in gilthead seabream (Sparus aurata L.) exposed to highly polluted marine sediments from Portman Bay (Spain). PG - 563-571 LID - S0045-6535(17)30187-X [pii] LID - 10.1016/j.chemosphere.2017.02.009 [doi] AB - Biomarkers have become crucial tools in modern environmental assessment as they can help to predict magnitude of pollution. The head-kidney (HK) and liver (hematopoietic and xenobiotic metabolism organs, respectively) are the key organs in all fish toxicological studies, although the skin has received less attention in this respect. The impact of two different types of polluted sediment collected from Portman Bay (Spain) on HK, liver and skin gene expression in gilthead seabream (Sparus aurata L.) exposed for two weeks to the sediments was determined by real time-PCR. The expression levels of different antioxidant enzyme genes [superoxide dismutase (sod) glutathione reductase (gr) and catalase (cat)] and immune-related genes [interleukin -1beta (il-1b), immunoglobulin M (igm), T-Cell receptor (tcr-b), cyclooxygenase-2 (cox-2), colony-stimulating factor 1-receptor (csf-1r) and hepcidin (hep)] was analysed. Expression varied depending on both the organ and gene studied: tcr-b, csf-1r and hep genes were down-regulated in HK, as were gr, tcr-b and il-1b in liver and gr and il-1b in skin, while cox-2 was up-regulated in skin after exposure to both sediments. Concomitantly, histopathological alterations were also studied in HK, liver and skin. While no significant changes were detected in skin cells of fish reared in aquaria with polluted sediments marked changes in the general morphology of HK and liver were observed, accompanied by a substantial degree of cell death and melano-macrophage centre disorganization. The present study suggests that the biomarkers studied in gilthead seabream could be useful for assessing the impact of pollution in coastal environments. CI - Copyright (c) 2017 Elsevier Ltd. All rights reserved. FAU - Ben Hamed, Said AU - Ben Hamed S AD - Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain; Laboratory of Microbiology, Research Unity of Biodiversity and Valorisation of Bioressources in Arid Zones, University of Gabes, Faculty of Sciences of Gabes, City Erriadh, 6072, Gabes, Tunisia. FAU - Guardiola, Francisco AU - Guardiola F AD - Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. FAU - Cuesta, Alberto AU - Cuesta A AD - Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. FAU - Martinez, Salvadora AU - Martinez S AD - Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Martinez-Sanchez, Maria Jose AU - Martinez-Sanchez MJ AD - Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Perez-Sirvent, Carmen AU - Perez-Sirvent C AD - Department of Agricultural Chemistry, Geology and Pedology, Faculty of Chemistry, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. FAU - Esteban, Maria Angeles AU - Esteban MA AD - Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address: aesteban@um.es. LA - eng PT - Journal Article DEP - 20170204 PL - England TA - Chemosphere JT - Chemosphere JID - 0320657 RN - 0 (Biomarkers) RN - 0 (Fish Proteins) RN - 0 (Hepcidins) RN - 0 (Immunoglobulin M) RN - 0 (Interleukin-1beta) RN - 0 (Receptors, Antigen, T-Cell, alpha-beta) RN - 0 (Water Pollutants, Chemical) RN - EC 1.11.1.6 (Catalase) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 1.8.1.7 (Glutathione Reductase) RN - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor) SB - IM MH - Animals MH - Bays MH - Biomarkers/metabolism MH - Catalase/genetics MH - Cyclooxygenase 2/genetics MH - Fish Proteins/genetics MH - Gene Expression Regulation/drug effects MH - Geologic Sediments MH - Glutathione Reductase/genetics MH - Head Kidney/drug effects/metabolism/pathology MH - Hepcidins/genetics MH - Immunoglobulin M/genetics MH - Interleukin-1beta/genetics MH - Liver/drug effects/metabolism/pathology MH - Receptor, Macrophage Colony-Stimulating Factor/genetics MH - Receptors, Antigen, T-Cell, alpha-beta/genetics MH - *Sea Bream/genetics/metabolism MH - Skin/anatomy & histology/drug effects/metabolism MH - Spain MH - Superoxide Dismutase/genetics MH - Water Pollutants, Chemical/*toxicity OTO - NOTNLM OT - Biomarkers OT - Gilthead seabream (Sparus aurata L.) OT - Histopathology OT - Immune genes OT - Oxidative stress OT - Pollution OT - Portman Bay EDAT- 2017/02/15 06:00 MHDA- 2017/05/13 06:00 CRDT- 2017/02/15 06:00 PHST- 2016/12/15 00:00 [received] PHST- 2017/02/02 00:00 [revised] PHST- 2017/02/02 00:00 [accepted] PHST- 2017/02/15 06:00 [pubmed] PHST- 2017/05/13 06:00 [medline] PHST- 2017/02/15 06:00 [entrez] AID - S0045-6535(17)30187-X [pii] AID - 10.1016/j.chemosphere.2017.02.009 [doi] PST - ppublish SO - Chemosphere. 2017 May;174:563-571. doi: 10.1016/j.chemosphere.2017.02.009. Epub 2017 Feb 4.