PMID- 28194716
OWN - NLM
STAT- MEDLINE
DCOM- 20170309
LR  - 20181113
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Linking)
VI  - 34
IP  - 3
DP  - 2017 Mar
TI  - Irradiation enhances dendritic cell potential antitumor activity by inducing 
      tumor cell expressing TNF-alpha.
PG  - 44
LID - 10.1007/s12032-016-0864-3 [doi]
AB  - Dendritic cells (DCs)-based tumor vaccines have shown to be the promising methods 
      for inducing therapeutic antitumor response. However, DCs alone rarely carry 
      curative antitumor activity, and the immunosuppressive microenvironment may 
      contribute to this defect of DC vaccinal function. Irradiation in combination 
      with DCs has been shown to promote immune-mediated tumor destruction in 
      preclinical studies. However, little is known about how irradiation alters the 
      tumor microenvironment, and what host pathways modulate the activity of 
      administrated DCs. In this study, BALB/c mice and the 4T1 breast cancer cell line 
      were used in a tumor-bearing model. The tumor-bearing mice were irradiated 
      locally up to 10 Gy for 3 consecutive days or a single dose of 30 Gy using a 
      cesium source. Studies of dynamic change of the tumor microenvironment in 
      irradiated versus untreated tumors revealed that there was no obvious change on 
      IL-10, IL-6 and TGF-beta expression or production, whereas increased TNF-alpha level 
      within the first 2 weeks of irradiation. The increased TNF-alpha level is exactly 
      right timing window for DCs injection, corresponding to the significant elevation 
      of intratumoral CD8(+) T infiltration and the regression of tumor size. With 
      attention to scheduling, combination X-ray with DCs i.t. injection may offer a 
      practical strategy to improve treatment outcomes.
FAU - Chang, Lijia
AU  - Chang L
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China.
FAU - Zhang, Zhengzheng
AU  - Zhang Z
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China.
FAU - Chen, Fang
AU  - Chen F
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China.
FAU - Zhang, Wen
AU  - Zhang W
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China.
FAU - Song, Shuang
AU  - Song S
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China.
FAU - Song, Shuxia
AU  - Song S
AD  - Department of Immunology and Key Laboratory of Immune Mechanism and Intervention 
      on Serious Disease, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017, People's Republic of China. songsxmy@hebmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170213
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology/*radiation effects
MH  - Female
MH  - Immunotherapy, Adoptive/*methods
MH  - Lymphocytes, Tumor-Infiltrating/immunology/radiation effects
MH  - Mammary Neoplasms, Experimental/*immunology/*therapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Random Allocation
MH  - T-Lymphocytes, Cytotoxic/immunology/radiation effects
MH  - Tumor Microenvironment/immunology/radiation effects
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/immunology
OTO - NOTNLM
OT  - DC vaccine
OT  - Irradiation
OT  - Microenvironment
OT  - TNF-alpha
OT  - Tumor-bearing mice
EDAT- 2017/02/15 06:00
MHDA- 2017/03/10 06:00
CRDT- 2017/02/15 06:00
PHST- 2016/07/17 00:00 [received]
PHST- 2016/11/30 00:00 [accepted]
PHST- 2017/02/15 06:00 [entrez]
PHST- 2017/02/15 06:00 [pubmed]
PHST- 2017/03/10 06:00 [medline]
AID - 10.1007/s12032-016-0864-3 [pii]
AID - 10.1007/s12032-016-0864-3 [doi]
PST - ppublish
SO  - Med Oncol. 2017 Mar;34(3):44. doi: 10.1007/s12032-016-0864-3. Epub 2017 Feb 13.