PMID- 28197078
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5099 (Print)
IS  - 1662-5099 (Electronic)
IS  - 1662-5099 (Linking)
VI  - 10
DP  - 2017
TI  - Redundant Postsynaptic Functions of SynCAMs 1-3 during Synapse Formation.
PG  - 24
LID - 10.3389/fnmol.2017.00024 [doi]
LID - 24
AB  - Investigating the roles of synaptogenic adhesion molecules during synapse 
      formation has proven challenging, often due to compensatory functions between 
      additional family members. The synaptic cell adhesion molecules 1-3 (SynCAM1-3) 
      are expressed both pre- and postsynaptically, share highly homologous domains and 
      are synaptogenic when ectopically presented to neurons; yet their endogenous 
      functions during synaptogenesis are unclear. Here we report that SynCAM1-3 are 
      functionally redundant and collectively necessary for synapse formation in 
      cultured hippocampal neurons. Only triple knockdown (KD) of SynCAM1-3 using 
      highly efficient, chained artificial microRNAs (amiRNAs) reduced synapse density 
      and increased synapse area. Electrophysiological recordings of quantal release 
      events supported an increase in synapse size caused by SynCAM1-3 depletion. 
      Furthermore, a combinatorial, mosaic lentiviral approach comparing wild type (WT) 
      and SynCAM1-3 KD neurons in the same culture demonstrate that SynCAM1-3 set 
      synapse number and size through postsynaptic mechanisms. The results demonstrate 
      that the redundancy between SynCAM1-3 has concealed their synaptogenic function 
      at the postsynaptic terminal.
FAU - Fowler, Daniel K
AU  - Fowler DK
AD  - Department of Biology, Institute of Neuroscience, University of OregonEugene, OR, 
      USA; Department of Integrative Physiology and Neuroscience, Washington State 
      UniversityPullman, WA, USA.
FAU - Peters, James H
AU  - Peters JH
AD  - Department of Integrative Physiology and Neuroscience, Washington State 
      University Pullman, WA, USA.
FAU - Williams, Carly
AU  - Williams C
AD  - Department of Biology, Institute of Neuroscience, University of Oregon Eugene, 
      OR, USA.
FAU - Washbourne, Philip
AU  - Washbourne P
AD  - Department of Biology, Institute of Neuroscience, University of Oregon Eugene, 
      OR, USA.
LA  - eng
GR  - R01 DK092651/DK/NIDDK NIH HHS/United States
GR  - T32 HD007348/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20170131
PL  - Switzerland
TA  - Front Mol Neurosci
JT  - Frontiers in molecular neuroscience
JID - 101477914
PMC - PMC5281628
OTO - NOTNLM
OT  - SynCAM
OT  - adhesion
OT  - artificial miRNA
OT  - hippocampus
OT  - knockdown
OT  - mosaic
OT  - redundancy
OT  - synapse formation
EDAT- 2017/02/16 06:00
MHDA- 2017/02/16 06:01
PMCR- 2017/01/01
CRDT- 2017/02/16 06:00
PHST- 2016/12/07 00:00 [received]
PHST- 2017/01/17 00:00 [accepted]
PHST- 2017/02/16 06:00 [entrez]
PHST- 2017/02/16 06:00 [pubmed]
PHST- 2017/02/16 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fnmol.2017.00024 [doi]
PST - epublish
SO  - Front Mol Neurosci. 2017 Jan 31;10:24. doi: 10.3389/fnmol.2017.00024. eCollection 
      2017.