PMID- 28198372
OWN - NLM
STAT- MEDLINE
DCOM- 20181012
LR  - 20240324
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Feb 15
TI  - Novel characteristics of the avian gyrovirus 2 genome.
PG  - 41068
LID - 10.1038/srep41068 [doi]
LID - 41068
AB  - Avian gyrovirus 2 (AGV2) was the second member of the viral genus Cyclovirus to 
      be discovered. This virus poses a significant potential threat to humans and 
      poultry due to its global dissemination and infectiousness. We used three 
      overlapping polymerase chain reactions (PCRs) to map the whole genome of AGV2. We 
      then modelled the evolutionary history of these novel sequence data in the 
      context of related sequences from GenBank. We analysed the viral protein 
      characteristics of the different phylogenetic groups and explored differences in 
      evolutionary trends between Chinese strains and strains from other countries. We 
      obtained 17 avian-sourced AGV2 whole genomes from different regions of China from 
      2015 to 2016. Phylogenetic analyses of these Chinese AGV2 sequences and related 
      sequences produced four distinct groups (A-D) with significant bootstrap values. 
      We also built phylogenies using predicted viral protein sequences. We found a 
      potential hypervariable region in VP1 at sites 288-314, and we identified the 
      amino acid changes responsible for the distinct VP2 and VP3 groups. Three new 
      motifs in the AGV2 5'-UTR direct repeat (DR) region were discovered and grouped. 
      The novel characteristics and diverse research on the AGV2 genome provide a 
      valuable framework for additional research.
FAU - Yao, Shuai
AU  - Yao S
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
AD  - Northeast Agricultural University, 150030, Harbin, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Tuo, Tianbei
AU  - Tuo T
AD  - Northeast Agricultural University, 150030, Harbin, China.
FAU - Han, Chunyan
AU  - Han C
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
AD  - Northeast Forestry University, 150040, Harbin, China.
FAU - Gao, Yulong
AU  - Gao Y
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Qi, Xiaole
AU  - Qi X
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Zhang, Yanping
AU  - Zhang Y
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Liu, Changjun
AU  - Liu C
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Gao, Honglei
AU  - Gao H
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Wang, Yongqiang
AU  - Wang Y
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
FAU - Wang, Xiaomei
AU  - Wang X
AD  - Division of Avian Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of 
      Agricultural Sciences, Harbin, 150069, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170215
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Capsid Proteins)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Evolution
MH  - Capsid Proteins/chemistry/classification/genetics
MH  - Chickens
MH  - DNA, Viral/genetics/metabolism
MH  - *Genome, Viral
MH  - Gyrovirus/classification/*genetics/isolation & purification
MH  - Nucleotide Motifs
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Poultry Diseases/virology
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
PMC - PMC5309784
COIS- The authors declare no competing financial interests.
EDAT- 2017/02/16 06:00
MHDA- 2018/10/13 06:00
PMCR- 2017/02/15
CRDT- 2017/02/16 06:00
PHST- 2016/09/15 00:00 [received]
PHST- 2016/12/13 00:00 [accepted]
PHST- 2017/02/16 06:00 [entrez]
PHST- 2017/02/16 06:00 [pubmed]
PHST- 2018/10/13 06:00 [medline]
PHST- 2017/02/15 00:00 [pmc-release]
AID - srep41068 [pii]
AID - 10.1038/srep41068 [doi]
PST - epublish
SO  - Sci Rep. 2017 Feb 15;7:41068. doi: 10.1038/srep41068.