PMID- 28198451
OWN - NLM
STAT- MEDLINE
DCOM- 20181024
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Feb 15
TI  - Elevated prevalence of Helicobacter species and virulence factors in 
      opisthorchiasis and associated hepatobiliary disease.
PG  - 42744
LID - 10.1038/srep42744 [doi]
LID - 42744
AB  - Recent reports suggest that Opisthorchis viverrini serves as a reservoir of 
      Helicobacter and implicate Helicobacter in pathogenesis of 
      opisthorchiasis-associated cholangiocarcinoma (CCA). Here, 553 age-sex matched 
      cases and controls, 293 and 260 positive and negative for liver fluke O. 
      viverrini eggs, of residents in Northeastern Thailand were investigated for 
      associations among infection with liver fluke, Helicobacter and hepatobiliary 
      fibrosis. The prevalence of H. pylori infection was higher in O. 
      viverrini-infected than uninfected participants. H. pylori bacterial load 
      correlated positively with intensity of O. viverrini infection, and participants 
      with opisthorchiasis exhibited higher frequency of virulent cagA-positive H. 
      pylori than those free of fluke infection. Genotyping of cagA from feces of both 
      infected and uninfected participants revealed that the AB genotype accounted for 
      78% and Western type 22%. Participants infected with O. viverrini exhibited 
      higher prevalence of typical Western type (EPIYA ABC) and variant AB'C type 
      (EPIYT B) CagA. Multivariate analyses among H. pylori virulence genes and 
      severity of hepatobiliary disease revealed positive correlations between biliary 
      periductal fibrosis during opisthorchiasis and CagA and CagA with CagA 
      multimerization (CM) sequence-positive H. pylori. These findings support the 
      hypothesis that H. pylori contributes to the pathogenesis of chronic 
      opisthorchiasis and specifically to opisthorchiasis-associated CCA.
FAU - Deenonpoe, Raksawan
AU  - Deenonpoe R
AD  - Tropical Disease Research Laboratory, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
FAU - Mairiang, Eimorn
AU  - Mairiang E
AD  - Departments of Radiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
FAU - Mairiang, Pisaln
AU  - Mairiang P
AD  - Departments of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
FAU - Pairojkul, Chawalit
AU  - Pairojkul C
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
FAU - Chamgramol, Yaovalux
AU  - Chamgramol Y
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
FAU - Rinaldi, Gabriel
AU  - Rinaldi G
AD  - Department of Microbiology, Immunology and Tropical Medicine, and Research Center 
      for Neglected Tropical Diseases of Poverty, School of Medicine &Health Sciences, 
      The George Washington University, Washington, DC, 20037, USA.
FAU - Loukas, Alex
AU  - Loukas A
AD  - Centre for Biodiscovery and Molecular Development of Therapeutics, Australian 
      Institute of Tropical Health &Medicine, James Cook University, Cairns, 
      Queensland, 4878, Australia.
FAU - Brindley, Paul J
AU  - Brindley PJ
AD  - Department of Microbiology, Immunology and Tropical Medicine, and Research Center 
      for Neglected Tropical Diseases of Poverty, School of Medicine &Health Sciences, 
      The George Washington University, Washington, DC, 20037, USA.
FAU - Sripa, Banchob
AU  - Sripa B
AD  - Tropical Disease Research Laboratory, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      40002, Thailand.
LA  - eng
GR  - P50 AI098639/AI/NIAID NIH HHS/United States
GR  - R01 CA164719/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170215
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (cagA protein, Helicobacter pylori)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antigens, Bacterial/genetics/metabolism
MH  - Bacterial Proteins/genetics/metabolism
MH  - Biliary Tract Diseases/*microbiology/parasitology
MH  - Coinfection/*microbiology/parasitology
MH  - Female
MH  - Helicobacter/*pathogenicity
MH  - Helicobacter Infections/*microbiology/parasitology
MH  - Humans
MH  - Liver Cirrhosis/*microbiology/parasitology
MH  - Male
MH  - Middle Aged
MH  - Opisthorchiasis/*microbiology/parasitology
PMC - PMC5309894
COIS- The authors declare no competing financial interests.
EDAT- 2017/02/16 06:00
MHDA- 2018/10/26 06:00
PMCR- 2017/02/15
CRDT- 2017/02/16 06:00
PHST- 2016/11/01 00:00 [received]
PHST- 2017/01/12 00:00 [accepted]
PHST- 2017/02/16 06:00 [entrez]
PHST- 2017/02/16 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
PHST- 2017/02/15 00:00 [pmc-release]
AID - srep42744 [pii]
AID - 10.1038/srep42744 [doi]
PST - epublish
SO  - Sci Rep. 2017 Feb 15;7:42744. doi: 10.1038/srep42744.