PMID- 28199692
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20240326
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 68
IP  - 5
DP  - 2017 Feb 1
TI  - Cellular localization of the Arabidopsis class 2 phytoglobin influences somatic 
      embryogenesis.
PG  - 1013-1023
LID - 10.1093/jxb/erx003 [doi]
AB  - Mutation of phytoglobin 2 (Pgb2) increases the number of somatic embryos in 
      Arabidopsis. To assess the effects of the cellular localization of Pgb2 on embryo 
      formation, an inducible system expressing a fusion protein consisting of Pgb2 
      linked to the steroid-binding domain of the rat glucocorticoid receptor (GR) was 
      introduced in a pgb2 mutant line lacking the ability to express Pgb2. In this 
      transgenic system, Pgb2 remains in the cytoplasm but migrates into the nucleus 
      upon exposure to dexamethasone (DEX). Pgb2 retention in the cytoplasm, in the 
      absence of DEX, increased the number of somatic embryos and reduced the 
      expression of MYC2 - an inhibitor of the synthesis of auxin, which is the 
      inductive signal for embryogenesis. Removal of DEX also induced the expression of 
      several genes involved in the biosynthesis of tryptophan and the auxin, 
      indole-3-acetic acid (IAA). These genes included: tryptophan synthase-alpha subunit 
      (TSA1) and tryptophan synthase-beta subunit (TSB1), which are involved in the 
      synthesis of tryptophan, cytochrome P450 CYP79B2 (CYP79B2) and amidase 1 (AMI1), 
      which participate in the formation of IAA via indole-3-acetaldoxime, and several 
      members of the YUCCA family, including YUC1 and 4, which are also required for 
      IAA synthesis. Retention of Pgb2 in the cytoplasm by removal of DEX increased the 
      staining pattern of IAA along the cotyledons of the explants generating 
      embryogenic tissue. Staining for IAA decreased when Pgb2 translocated into the 
      nucleus in response to the application of DEX. Collectively, these results 
      suggest that the presence of Pgb2 in the cytoplasm, but not in the nucleus, 
      phenocopies the effects of Pgb2 mutation in inducing somatic embryogenesis.
CI  - (c) The Author 2017. Published by Oxford University Press on behalf of the Society 
      for Experimental Biology.
FAU - Godee, Cara
AU  - Godee C
AD  - Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, 
      Canada.
FAU - Mira, Mohamed M
AU  - Mira MM
AD  - Permanent address: Department of Botany, Faculty of Science, Tanta University, 
      Tanta, Egypt 31527.
FAU - Wally, Owen
AU  - Wally O
AD  - Agriculture and Agri-Food Canada/Government of Canada, Harrow Research and 
      Development Centre, RR #2, 2585 County Rd. 20, Harrow, ON N0R 1G0, Canada.
FAU - Hill, Robert D
AU  - Hill RD
AD  - Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, 
      Canada.
FAU - Stasolla, Claudio
AU  - Stasolla C
AD  - Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, 
      Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (phytoglobin 2 protein, Arabidopsis)
RN  - 6U1S09C61L (indoleacetic acid)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 8DUH1N11BX (Tryptophan)
SB  - IM
MH  - Arabidopsis/embryology/*genetics/metabolism
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Dexamethasone/administration & dosage
MH  - Gene Expression Regulation, Plant/*drug effects
MH  - Indoleacetic Acids/*metabolism
MH  - *Plant Somatic Embryogenesis Techniques
MH  - Tryptophan/*metabolism
PMC - PMC5441859
OTO - NOTNLM
OT  - Auxin
OT  - dexamethasone
OT  - phytoglobin
OT  - somatic embryogenesis.
EDAT- 2017/02/16 06:00
MHDA- 2017/11/29 06:00
PMCR- 2017/02/11
CRDT- 2017/02/16 06:00
PHST- 2017/02/16 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/02/16 06:00 [entrez]
PHST- 2017/02/11 00:00 [pmc-release]
AID - 2986655 [pii]
AID - erx003 [pii]
AID - 10.1093/jxb/erx003 [doi]
PST - ppublish
SO  - J Exp Bot. 2017 Feb 1;68(5):1013-1023. doi: 10.1093/jxb/erx003.