PMID- 28199745
OWN - NLM
STAT- MEDLINE
DCOM- 20170804
LR  - 20220408
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 85
IP  - 5
DP  - 2017 May
TI  - Bacterial N-formyl Peptides Reduce PMA- and Escherichia coli-Induced Neutrophil 
      Respiratory Burst in Term Neonates and Adults.
PG  - 365-371
LID - 10.1111/sji.12537 [doi]
AB  - Neutrophil migration and respiratory burst are the prerequisite for efficient 
      first line defense against invading microorganisms. However, migration and 
      respiratory burst can be compromised in adults and especially in newborn infants, 
      where sustained neutrophil accumulation, uncontrolled burst and reduced 
      scavenging of ROS might cause inadvertent tissue damage due to uncontrolled 
      inflammation. The aim of this study was to investigate the modulatory effect of 
      the chemoattractants formyl-methionyl-leucyl-phenylalanine (fMLP) and IL-8 on 
      respiratory burst in neutrophils from term newborn infants and adults. Whole 
      blood from the umbilical cord of 17 healthy term newborn infants delivered by 
      caesarean section and from 17 healthy adults as reference was preincubated with 
      fMLP or IL-8 and stimulated with PMA or Escherichia coli bacteria. Respiratory 
      burst was quantified by flow cytometry analysis of dihydrorhodamine 123 
      fluorescence. fMLP reduced the PMA-induced respiratory burst of neutrophils from 
      newborn infants and adults by 12% and 21%, respectively (P < 0.05). 
      E. coli-induced burst was also reduced by fMLP in neutrophils from newborn 
      infants (10%; P < 0.01) and adults (6%; P < 0.05). No such changes were observed 
      with IL-8. Similar respiratory burst in response to single stimulus with PMA or 
      E. coli was observed in both newborn infants and adults. fMLP reduced PMA- and 
      E. coli-induced respiratory burst of neutrophils in whole blood from term newborn 
      infants as well as in adults. The reduced respiratory burst by fMLP might be a 
      mechanism to reduce the detrimental effects of uncontrolled inflammation during 
      neutrophil migration.
CI  - (c) 2017 The Foundation for the Scandinavian Journal of Immunology.
FAU - Stalhammar, M E
AU  - Stalhammar ME
AUID- ORCID: 0000-0003-1362-1714
AD  - Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
FAU - Douhan Hakansson, L
AU  - Douhan Hakansson L
AD  - Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
FAU - Sindelar, R
AU  - Sindelar R
AD  - Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Interleukin-8)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
CIN - Scand J Immunol. 2017 Aug;86(2):118. PMID: 28547744
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Dose-Response Relationship, Drug
MH  - Escherichia coli/*growth & development/physiology
MH  - Flow Cytometry
MH  - Humans
MH  - Infant, Newborn
MH  - Interleukin-8/pharmacology
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*drug effects/metabolism/microbiology
MH  - Respiratory Burst/*drug effects
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Young Adult
EDAT- 2017/02/16 06:00
MHDA- 2017/08/05 06:00
CRDT- 2017/02/16 06:00
PHST- 2016/12/16 00:00 [received]
PHST- 2017/02/01 00:00 [accepted]
PHST- 2017/02/16 06:00 [pubmed]
PHST- 2017/08/05 06:00 [medline]
PHST- 2017/02/16 06:00 [entrez]
AID - 10.1111/sji.12537 [doi]
PST - ppublish
SO  - Scand J Immunol. 2017 May;85(5):365-371. doi: 10.1111/sji.12537.