PMID- 28202040
OWN - NLM
STAT- MEDLINE
DCOM- 20170823
LR  - 20181113
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 14
IP  - 1
DP  - 2017 Feb 15
TI  - Subcellular localization and interactions of Infectious Salmon Anemia Virus 
      (ISAV) M1 and NEP as well as host Hsc70.
PG  - 30
LID - 10.1186/s12985-017-0702-z [doi]
LID - 30
AB  - BACKGROUND: Infectious salmon anemia virus (ISAV) is an important fish pathogen 
      that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of 
      eight single-stranded, negative-sense RNA segments. The six largest segments 
      contain one open reading frame (ORF) each, and encode three polymerase proteins, 
      nucleoprotein, fusion protein, and hemagglutinin esterase protein. The two 
      smallest segments contain more than one ORF each. The segment 7 encodes 
      non-structural protein 1 (NS1) and nuclear export protein (NEP), while segment 8 
      encodes matrix protein 1 and 2 (M1 and M2). NS1 and M2 have been well known as 
      antagonist of type I interferon. However, little is known about the 
      characterization of M1 or NEP. In addition, heat shock cognate 70 (Hsc70) has 
      been reported to interact with M1 and NEP of influenza viruses for the export of 
      viral ribonucleoprotein (vRNP) via vRNP-M1-NEP complex, the goal of this study 
      therefore was to characterize the subcellular localization and interactions of 
      ISAV M1 and NEP as well as cellular Hsc70. RESULTS: When M1, NEP, and Hsc70 were 
      individually expressed in the stripped snakehead (SSN-1) cells, we found that M1 
      protein was localized in both cytosol and nucleus of the cells, NEP was localized 
      only in the cytosol and accumulated adjacent to the nucleus, while Hsc70 was 
      localized throughout the cytosol, but not in the nucleus. However, when two of 
      them were co-expressed, we found that both M1 and Hsc70 were co-localized with 
      NEP in the cytosol and accumulated adjacent to the nucleus, while M1 and Hsc70 
      were still localized as they were expressed individually. Furthermore, pull-down 
      assay was performed and showed that NEP could interact with both M1 and Hsc70, 
      and M1-Hsc70 interaction was also observed although the interaction was weaker 
      than that of NEP-Hsc70. CONCLUSION: Our study characterized the subcellular 
      localization and interactions of three proteins including M1 and NEP of ISAV, and 
      Hsc70. These data will help towards a better understanding of the life cycle of 
      ISAV, especially the process of vRNP export.
FAU - Zhang, Wenting
AU  - Zhang W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of 
      China.
FAU - Cai, Chengzhi
AU  - Cai C
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of 
      China.
FAU - Lin, Li
AU  - Lin L
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Huazhong 
      Agricultural University, Wuhan, Hubei, 430070, China.
FAU - Tao, Yizhi Jane
AU  - Tao YJ
AD  - Department of Biosciences, Rice University, Houston, TX, USA.
FAU - Jin, Meilin
AU  - Jin M
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of 
      China. jml8328@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170215
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - HSC70 Heat-Shock Proteins/*metabolism
MH  - *Host-Pathogen Interactions
MH  - Isavirus/*physiology
MH  - Protein Binding
MH  - *Protein Interaction Mapping
MH  - Salmo salar
MH  - Viral Proteins/*metabolism
PMC - PMC5310077
OTO - NOTNLM
OT  - Heat shock cognate 70
OT  - Infectious salmon anemia virus
OT  - Matrix protein
OT  - Nuclear export protein
OT  - Protein interaction
OT  - Subcellular localization
EDAT- 2017/02/17 06:00
MHDA- 2017/08/24 06:00
PMCR- 2017/02/15
CRDT- 2017/02/17 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/02/08 00:00 [accepted]
PHST- 2017/02/17 06:00 [entrez]
PHST- 2017/02/17 06:00 [pubmed]
PHST- 2017/08/24 06:00 [medline]
PHST- 2017/02/15 00:00 [pmc-release]
AID - 10.1186/s12985-017-0702-z [pii]
AID - 702 [pii]
AID - 10.1186/s12985-017-0702-z [doi]
PST - epublish
SO  - Virol J. 2017 Feb 15;14(1):30. doi: 10.1186/s12985-017-0702-z.