PMID- 28203238
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 8
DP  - 2017
TI  - Differential Ability of Bovine Antimicrobial Cathelicidins to Mediate Nucleic 
      Acid Sensing by Epithelial Cells.
PG  - 59
LID - 10.3389/fimmu.2017.00059 [doi]
LID - 59
AB  - Cathelicidins encompass a family of cationic peptides characterized by 
      antimicrobial activity and other functions, such as the ability to enhance the 
      sensing of nucleic acids by the innate immune system. The present study aimed to 
      investigate the ability of the bovine cathelicidins indolicidin, bactenecin 
      (Bac)1, Bac5, bovine myeloid antimicrobial peptide (BMAP)-27, BMAP-28, and 
      BMAP-34 to inhibit the growth of bacteria and to enhance the sensing of nucleic 
      acid by the host's immune system. BMAP-27 was the most effective at killing 
      Staphylococcus aureus, Streptococcus uberis, and Escherichia coli, and this was 
      dependent on its amphipathic structure and cationic charge. Although most 
      cathelicidins possessed DNA complexing activity, only the alpha-helical BMAP 
      cathelicidins and the cysteine-rich disulfide-bridged Bac1 were able to enhance 
      the sensing of nucleic acids by primary epithelial cells. We also compared these 
      responses with those mediated by neutrophils. Activation of neutrophils with 
      phorbol myristate acetate resulted in degranulation and release of cathelicidins 
      as well as bactericidal activity in the supernatants. However, only supernatants 
      from unstimulated neutrophils were able to promote nucleic acid sensing in 
      epithelial cells. Collectively, the present data support a role for certain 
      bovine cathelicidins in helping the innate immune system to sense nucleic acids. 
      The latter effect is observed at concentrations clearly below those required for 
      direct antimicrobial functions. These findings are relevant in development of 
      future strategies to promote protection at mucosal surfaces against pathogen 
      invasion.
FAU - Baumann, Arnaud
AU  - Baumann A
AD  - Institute of Virology and Immunology , Bern , Switzerland.
FAU - Kiener, Mirjam Susanna
AU  - Kiener MS
AD  - Institute of Virology and Immunology , Bern , Switzerland.
FAU - Haigh, Brendan
AU  - Haigh B
AD  - AgResearch, Ruakura Research Centre , Hamilton , New Zealand.
FAU - Perreten, Vincent
AU  - Perreten V
AD  - Vetsuisse Faculty, Department of Infectious Diseases and Pathobiology, Institute 
      of Veterinary Bacteriology, University of Bern , Bern , Switzerland.
FAU - Summerfield, Artur
AU  - Summerfield A
AD  - Institute of Virology and Immunology, Bern, Switzerland; Vetsuisse Faculty, 
      Department of Infectious Diseases and Pathobiology, University of Bern, Bern, 
      Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20170201
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC5285380
OTO - NOTNLM
OT  - Mx1
OT  - antimicrobial cationic peptides
OT  - bacterial infection
OT  - cathelicidin
OT  - cattle
OT  - epithelial cells
OT  - nucleic acid sensing
OT  - type I IFN
EDAT- 2017/02/17 06:00
MHDA- 2017/02/17 06:01
PMCR- 2017/01/01
CRDT- 2017/02/17 06:00
PHST- 2016/08/31 00:00 [received]
PHST- 2017/01/16 00:00 [accepted]
PHST- 2017/02/17 06:00 [entrez]
PHST- 2017/02/17 06:00 [pubmed]
PHST- 2017/02/17 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2017.00059 [doi]
PST - epublish
SO  - Front Immunol. 2017 Feb 1;8:59. doi: 10.3389/fimmu.2017.00059. eCollection 2017.