PMID- 28209486 OWN - NLM STAT- MEDLINE DCOM- 20170526 LR - 20220129 IS - 1090-2422 (Electronic) IS - 0014-4827 (Print) IS - 0014-4827 (Linking) VI - 352 IP - 2 DP - 2017 Mar 15 TI - Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, beta-catenin and urokinase-plasminogen activator (uPA). PG - 281-292 LID - S0014-4827(17)30064-2 [pii] LID - 10.1016/j.yexcr.2017.02.019 [doi] AB - A hallmark of cancer cells is their ability to invade surrounding tissues and form metastases. Cell-extracellular matrix (ECM)-adhesion proteins are crucial in metastasis, connecting tumor ECM with actin cytoskeleton thus enabling cells to respond to mechanical cues. Vasodilator-stimulated phosphoprotein (VASP) is an actin-polymerization regulator which interacts with cell-ECM adhesion protein Migfilin, and regulates cell migration. We compared VASP expression in MCF-7 and MDA-MB-231 breast cancer (BC) cells and found that more invasive MDA-MB-231 cells overexpress VASP. We then utilized a 3-dimensional (3D) approach to study metastasis in MDA-MB-231 cells using a system that considers mechanical forces exerted by the ECM. We prepared 3D collagen I gels of increasing concentration, imaged them by atomic force microscopy, and used them to either embed cells or tumor spheroids, in the presence or absence of VASP. We show, for the first time, that VASP silencing downregulated Migfilin, beta-catenin and urokinase plasminogen activator both in 2D and 3D, suggesting a matrix-independent mechanism. Tumor spheroids lacking VASP demonstrated impaired invasion, indicating VASP's involvement in metastasis, which was corroborated by Kaplan-Meier plotter showing high VASP expression to be associated with poor remission-free survival in lymph node-positive BC patients. Hence, VASP may be a novel BC metastasis biomarker. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Gkretsi, Vasiliki AU - Gkretsi V AD - Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, University of Cyprus, Cyprus. FAU - Stylianou, Andreas AU - Stylianou A AD - Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, University of Cyprus, Cyprus. FAU - Stylianopoulos, Triantafyllos AU - Stylianopoulos T AD - Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, University of Cyprus, Cyprus. Electronic address: tstylian@ucy.ac.cy. LA - eng GR - 336839/ERC_/European Research Council/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170214 PL - United States TA - Exp Cell Res JT - Experimental cell research JID - 0373226 RN - 0 (Cell Adhesion Molecules) RN - 0 (Cytoskeletal Proteins) RN - 0 (FBLIM1 protein, human) RN - 0 (Microfilament Proteins) RN - 0 (Phosphoproteins) RN - 0 (beta Catenin) RN - 0 (vasodilator-stimulated phosphoprotein) RN - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator) SB - IM MH - Breast Neoplasms/*metabolism MH - Cell Adhesion Molecules/genetics/*metabolism MH - Cytoskeletal Proteins/genetics/*metabolism MH - *Down-Regulation MH - Humans MH - MCF-7 Cells MH - Microfilament Proteins/genetics/*metabolism MH - Phosphoproteins/genetics/*metabolism MH - Spheroids, Cellular MH - Urokinase-Type Plasminogen Activator/genetics/*metabolism MH - beta Catenin/genetics/*metabolism PMC - PMC5349498 MID - EMS71607 OTO - NOTNLM OT - Extracellular matrix OT - Migfilin OT - Tumor spheroid invasion OT - Urokinase plasminogen activator OT - Vasodilator stimulated phosphoprotein EDAT- 2017/02/18 06:00 MHDA- 2017/05/27 06:00 PMCR- 2018/03/15 CRDT- 2017/02/18 06:00 PHST- 2016/09/02 00:00 [received] PHST- 2017/01/18 00:00 [revised] PHST- 2017/02/13 00:00 [accepted] PHST- 2017/02/18 06:00 [pubmed] PHST- 2017/05/27 06:00 [medline] PHST- 2017/02/18 06:00 [entrez] PHST- 2018/03/15 00:00 [pmc-release] AID - S0014-4827(17)30064-2 [pii] AID - 10.1016/j.yexcr.2017.02.019 [doi] PST - ppublish SO - Exp Cell Res. 2017 Mar 15;352(2):281-292. doi: 10.1016/j.yexcr.2017.02.019. Epub 2017 Feb 14.