PMID- 28209776
OWN - NLM
STAT- MEDLINE
DCOM- 20180412
LR  - 20231115
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 114
IP  - 10
DP  - 2017 Mar 7
TI  - Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering 
      dendrites.
PG  - E1986-E1995
LID - 10.1073/pnas.1607462114 [doi]
AB  - Neurons receive a multitude of synaptic inputs along their dendritic arbor, but 
      how this highly heterogeneous population of synaptic compartments is spatially 
      organized remains unclear. By measuring N-methyl-d-aspartic acid receptor 
      (NMDAR)-driven calcium responses in single spines, we provide a spatial map of 
      synaptic calcium signals along dendritic arbors of hippocampal neurons and relate 
      this to measures of synapse structure. We find that quantal NMDAR calcium signals 
      increase in amplitude as they approach a thinning dendritic tip end. Based on a 
      compartmental model of spine calcium dynamics, we propose that this biased 
      distribution in calcium signals is governed by a gradual, distance-dependent 
      decline in spine size, which we visualized using serial block-face scanning 
      electron microscopy. Our data describe a cell-autonomous feature of principal 
      neurons, where tapering dendrites show an inverse distribution of spine size and 
      NMDAR-driven calcium signals along dendritic trees, with important implications 
      for synaptic plasticity rules and spine function.
FAU - Walker, Alison S
AU  - Walker AS
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - Neves, Guilherme
AU  - Neves G
AUID- ORCID: 0000-0001-6463-6997
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - Grillo, Federico
AU  - Grillo F
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - Jackson, Rachel E
AU  - Jackson RE
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - Rigby, Mark
AU  - Rigby M
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - O'Donnell, Cian
AU  - O'Donnell C
AUID- ORCID: 0000-0003-2031-9177
AD  - Department of Computer Science, University of Bristol, Bristol BS8 1UB, United 
      Kingdom.
FAU - Lowe, Andrew S
AU  - Lowe AS
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom.
FAU - Vizcay-Barrena, Gema
AU  - Vizcay-Barrena G
AD  - Centre for Ultrastructural Imaging, Kings College London, London SE1 1UL, United 
      Kingdom.
FAU - Fleck, Roland A
AU  - Fleck RA
AD  - Centre for Ultrastructural Imaging, Kings College London, London SE1 1UL, United 
      Kingdom.
FAU - Burrone, Juan
AU  - Burrone J
AD  - Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, 
      United Kingdom; juan.burrone@kcl.ac.uk.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - MR/N026063/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170216
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Signaling
MH  - Dendritic Spines/*metabolism/ultrastructure
MH  - Embryo, Mammalian
MH  - Female
MH  - Gene Expression
MH  - Hippocampus/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron
MH  - Microtomy
MH  - N-Methylaspartate/metabolism
MH  - Neuronal Plasticity
MH  - Pregnancy
MH  - Primary Cell Culture
MH  - Pyramidal Cells/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Synapses/physiology
PMC - PMC5347575
OTO - NOTNLM
OT  - NMDA
OT  - calcium
OT  - dendrite
OT  - dendritic spine
OT  - hippocampus
COIS- The authors declare no conflict of interest.
EDAT- 2017/02/18 06:00
MHDA- 2018/04/13 06:00
PMCR- 2017/09/07
CRDT- 2017/02/18 06:00
PHST- 2017/02/18 06:00 [pubmed]
PHST- 2018/04/13 06:00 [medline]
PHST- 2017/02/18 06:00 [entrez]
PHST- 2017/09/07 00:00 [pmc-release]
AID - 1607462114 [pii]
AID - 201607462 [pii]
AID - 10.1073/pnas.1607462114 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):E1986-E1995. doi: 
      10.1073/pnas.1607462114. Epub 2017 Feb 16.