PMID- 28211079
OWN - NLM
STAT- MEDLINE
DCOM- 20180411
LR  - 20180411
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 71
IP  - 1
DP  - 2017 Jul
TI  - GATA3 expression in triple-negative breast cancers.
PG  - 63-71
LID - 10.1111/his.13187 [doi]
AB  - AIMS: GATA-binding protein 3 (GATA3) is a well-studied transcription factor found 
      to be essential in the development of luminal breast epithelium and has been 
      identified in a variety of tumour types, including breast and urothelial 
      carcinomas, making it a useful immunohistochemistry marker in the diagnosis of 
      both primary and metastatic disease. METHODS AND RESULTS: We investigated GATA3 
      protein expression in a 106 primary triple-negative breast carcinomas (100 
      basal-like, six non-basal-like) using Cell Marque mouse monoclonal anti-GATA3 
      (L50-823). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) 
      was used to quantify mRNA expression in 22 triple-negative breast cancers (TNBCs) 
      (20 primary and two cell lines), four luminal (three primary and one cell line) 
      and five human epidermal growth factor receptor 2 (HER2) (four primary and one 
      cell line) amplified tumours. In 98 TNBCs where IHC was assessable, 47 (48%) had 
      a 1+ or greater staining with 20 (21%) having high GATA3 expression when using a 
      weighted scoring. CONCLUSION: Our study has demonstrated that GATA3 expression is 
      common in primary triple-negative breast carcinomas. It also suggests that 
      although GATA3 is an oestrogen receptor (ER) regulated gene, it still proves 
      useful in differentiating between primary and metastatic tumours in patients with 
      a history of breast cancer regardless of its molecular subtype.
CI  - (c) 2017 John Wiley & Sons Ltd.
FAU - Byrne, David J
AU  - Byrne DJ
AUID- ORCID: 0000-0002-9258-3252
AD  - Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, 
      Australia.
FAU - Deb, Siddhartha
AU  - Deb S
AD  - Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, 
      Australia.
FAU - Takano, Elena A
AU  - Takano EA
AD  - Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, 
      Australia.
FAU - Fox, Stephen B
AU  - Fox SB
AD  - Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, 
      Australia.
AD  - Department of Pathology, the University of Melbourne, Melbourne, Victoria, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20170419
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Female
MH  - GATA3 Transcription Factor/analysis/*biosynthesis
MH  - Humans
MH  - Middle Aged
MH  - Triple Negative Breast Neoplasms/*metabolism
OTO - NOTNLM
OT  - TNBC
OT  - GATA3
OT  - Immunohistochemistry
OT  - breast cancer
EDAT- 2017/02/18 06:00
MHDA- 2018/04/12 06:00
CRDT- 2017/02/18 06:00
PHST- 2016/09/07 00:00 [received]
PHST- 2017/02/14 00:00 [accepted]
PHST- 2017/02/18 06:00 [pubmed]
PHST- 2018/04/12 06:00 [medline]
PHST- 2017/02/18 06:00 [entrez]
AID - 10.1111/his.13187 [doi]
PST - ppublish
SO  - Histopathology. 2017 Jul;71(1):63-71. doi: 10.1111/his.13187. Epub 2017 Apr 19.