PMID- 2821233
OWN - NLM
STAT- MEDLINE
DCOM- 19871116
LR  - 20190510
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 383
DP  - 1987 Feb
TI  - Differential control of azurophilic and specific granule exocytosis in 
      Sendai-virus-permeabilized rabbit neutrophils.
PG  - 115-24
AB  - 1. Rabbit neutrophils were permeabilized by treatment with Sendai virus. This was 
      monitored by fluorescence measurement of the formation of the adduct of 
      deoxyribonucleic acid (DNA) with ethidium bromide. 2. On addition of Ca2+, 
      buffered (with EGTA) in the micromolar concentration range to the permeabilized 
      cells, secretion of beta-glucuronidase (marker of azurophilic granules) and 
      lysozyme (marker of specific granules) occurs. Lactate dehydrogenase (cytosol 
      marker) is retained. Half-maximal secretion of beta-glucuronidase occurs at 
      approximately pCa 6.3; lysozyme secretion occurs at approximately pCa 6.6. 3. 
      Secretion is dependent on the provision of nucleoside triphosphates to the 
      permeabilized cells. There is an absolute requirement for adenosine 
      5'-triphosphate (ATP) for the secretion of lysozyme, but beta-glucuronidase 
      secretion can be partly supported by other nucleoside triphosphates in the order 
      guanosine 5'-triphosphate (GTP) greater than uridine 5'-triphosphate (UTP) = 
      xanthosine 5'-triphosphate (XTP) greater than cytidine 5'-triphosphate (CTP). 4. 
      Secretion from both granules is complete within 10 min of adding Ca2+ to the 
      permeabilized cells. There is a delay before commencement of beta-glucuronidase 
      secretion of approximately half a minute; the secretion of lysozyme has no 
      measurable delay.
FAU - Barrowman, M M
AU  - Barrowman MM
AD  - Department of Experimental Pathology, University College London.
FAU - Cockcroft, S
AU  - Cockcroft S
FAU - Gomperts, B D
AU  - Gomperts BD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Purine Nucleotides)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Calcium/*pharmacology
MH  - Cell Membrane Permeability
MH  - Cytoplasmic Granules
MH  - *Exocytosis
MH  - Glucuronidase/metabolism
MH  - In Vitro Techniques
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Muramidase/metabolism
MH  - Neutrophils/metabolism/*physiology
MH  - Parainfluenza Virus 1, Human
MH  - Purine Nucleotides/metabolism
MH  - Rabbits
MH  - Time Factors
PMC - PMC1183060
EDAT- 1987/02/01 00:00
MHDA- 1987/02/01 00:01
PMCR- 1987/02/01
CRDT- 1987/02/01 00:00
PHST- 1987/02/01 00:00 [pubmed]
PHST- 1987/02/01 00:01 [medline]
PHST- 1987/02/01 00:00 [entrez]
PHST- 1987/02/01 00:00 [pmc-release]
AID - 10.1113/jphysiol.1987.sp016399 [doi]
PST - ppublish
SO  - J Physiol. 1987 Feb;383:115-24. doi: 10.1113/jphysiol.1987.sp016399.