PMID- 28212895
OWN - NLM
STAT- MEDLINE
DCOM- 20171106
LR  - 20181202
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 248
DP  - 2017 Jul 1
TI  - The influence of enteral obestatin administration to suckling rats on intestinal 
      contractility.
PG  - 69-78
LID - S0016-6480(17)30125-9 [pii]
LID - 10.1016/j.ygcen.2017.02.006 [doi]
AB  - This study investigated the effect of enteral administration of obestatin on the 
      contractility of whole-thickness preparations of duodenum and middle jejunum, as 
      well as on the morphology of the enteric nervous system (ENS). Suckling rats were 
      assigned to 3 groups (n=12) treated with: C-saline solution; LO-obestatin 
      (125nmol/kgb.wt); HO-obestatin (250nmol/kgb.wt). Saline solution or obestatin 
      were administered twice daily, from the 14th to the 21st day of life. Sections 
      were studied in an organ bath, for isometric recording in the presence of 
      acetylocholine (ACh), atropine (ATR) and tetradotoxin (TTX). Thickness of 
      intestinal muscularis layer, the number of interstitial cells of Cajal (ICC) were 
      measured in the paraffin sections. The immunodetection of Muscarinic 
      Acetylocholine Receptor 2 (M2 receptor) was performed in the intestinal segments. 
      In both intestinal segments HO treatment decreased the amplitude of spontaneous 
      contraction compared to that observed in the C group. In the middle jejunum, the 
      LO treatment also decreased the amplitude. TTX and ATR had no effect on amplitude 
      of spontaneous contraction in the jejunum of LO and HO-treated animals. Compared 
      to the C group, duodenal sections from HO animals and middle jejunum sections 
      from LO and HO groups displayed a lower amplitude in response to ACh and EFS 
      evoked contraction. An increase in the thickness of the muscularis layer was 
      observed in the duodenum of LO and HO groups whereas the number ICC did not 
      change significantly after treatment with obestatin. Moreover, the enteral 
      administration of obestatin did not effect significantly on the cytoplasmic 
      expression of M2 receptor in the jejunum. Our study demonstrated that enteral 
      administration of obestatin to suckling rats influences small intestine 
      contractility in the segment specific manner.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Slupecka, M
AU  - Slupecka M
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, Jablonna, Poland. Electronic address: 
      m.slupecka@ifzz.pl.
FAU - Grzesiak, P
AU  - Grzesiak P
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, Jablonna, Poland.
FAU - Kwiatkowski, J
AU  - Kwiatkowski J
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, Jablonna, Poland.
FAU - Gajewska, M
AU  - Gajewska M
AD  - Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw 
      University of Life Sciences, Warsaw, Poland.
FAU - Kuwahara, A
AU  - Kuwahara A
AD  - Laboratory of Physiology, Institute for Environmental Sciences and Graduate 
      School of Nutritional and Enviromental Science, University of Shizuoka, Japan.
FAU - Kato, I
AU  - Kato I
AD  - Department of Medical Biochemistry, Kobe Pharmaceutical University, Japan.
FAU - Wolinski, J
AU  - Wolinski J
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, Jablonna, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170216
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Ghrelin)
RN  - 0 (Receptor, Muscarinic M2)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Cell Count
MH  - Electric Stimulation
MH  - Enteral Nutrition
MH  - Female
MH  - Gastrointestinal Motility/drug effects/*physiology
MH  - Ghrelin/*administration & dosage/*pharmacology
MH  - Interstitial Cells of Cajal/cytology/drug effects
MH  - Intestines/drug effects/*physiology
MH  - Male
MH  - Muscle Contraction/*drug effects
MH  - Proto-Oncogene Proteins c-kit/metabolism
MH  - Rats
MH  - Receptor, Muscarinic M2
MH  - Tetrodotoxin/pharmacology
OTO - NOTNLM
OT  - Contractility
OT  - Intestine
OT  - Obestatin
OT  - Rat
EDAT- 2017/02/19 06:00
MHDA- 2017/11/07 06:00
CRDT- 2017/02/19 06:00
PHST- 2016/08/14 00:00 [received]
PHST- 2017/01/24 00:00 [revised]
PHST- 2017/02/13 00:00 [accepted]
PHST- 2017/02/19 06:00 [pubmed]
PHST- 2017/11/07 06:00 [medline]
PHST- 2017/02/19 06:00 [entrez]
AID - S0016-6480(17)30125-9 [pii]
AID - 10.1016/j.ygcen.2017.02.006 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2017 Jul 1;248:69-78. doi: 10.1016/j.ygcen.2017.02.006. Epub 
      2017 Feb 16.