PMID- 28213827
OWN - NLM
STAT- MEDLINE
DCOM- 20170720
LR  - 20200930
IS  - 1573-3017 (Electronic)
IS  - 0963-9292 (Print)
IS  - 0963-9292 (Linking)
VI  - 26
IP  - 3
DP  - 2017 Apr
TI  - Resistance to polycyclic aromatic hydrocarbon toxicity and associated 
      bioenergetic consequences in a population of Fundulus heteroclitus.
PG  - 435-448
LID - 10.1007/s10646-017-1775-6 [doi]
AB  - Several locations in the Elizabeth River, VA, USA are highly contaminated with 
      polycyclic aromatic hydrocarbons (PAHs) due to the release of creosote mixtures 
      from wood treatment facilities. Interestingly, some populations of Atlantic 
      killifish (Fundulus heteroclitus) inhabiting the Elizabeth River (ER) are 
      resistant to PAH-induced teratogenesis. However, evolutionary resistance to PAHs 
      due to chronic PAH exposure is associated with reduced fitness and increased 
      susceptibility to other environmental stressors in at least one PAH-resistant ER 
      killifish population. More specifically, wild-caught and first generation 
      PAH-resistant juvenile killifish have altered metabolic demands when compared to 
      non-resistant fish. Herein, we investigated this association further by examining 
      a previously under-studied population captured from the creosote-contaminated 
      site Republic Creosoting (Rep). We assessed PAH toxicity and effects on energy 
      metabolism in Rep killifish in comparison with killifish from the reference site 
      Kings Creek (KC). Following exposures to simple and complex PAH mixtures, Rep 
      killifish exhibited several phenotypes associated with PAH resistance including 
      decreased incidences of developmental cardiovascular deformities and recalcitrant 
      cytochrome P450 1A (CYP1A) activity. We evaluated bioenergetics in killifish 
      embryos throughout development and found elevated basal oxygen consumption rates 
      in Rep embryos relative to KC embryos. Furthermore, juvenile F1 Rep fish had 
      significantly lower maximal metabolic rates and aerobic scopes than KC juveniles. 
      These results suggest that populations of killifish that have adapted or evolved 
      to withstand the toxicity associated with PAHs consequently have altered 
      energetic metabolism or demands. Such consequences could result in an enhanced 
      vulnerability to other environmental and anthropogenic stressors in PAH-resistant 
      killifish.
FAU - Lindberg, C D
AU  - Lindberg CD
AD  - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA. 
      cdl27@duke.edu.
AD  - Integrated Toxicology and Environmental Health Program, Duke University, Durham, 
      NC, 27708, USA. cdl27@duke.edu.
FAU - Jayasundara, N
AU  - Jayasundara N
AD  - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA.
FAU - Kozal, J S
AU  - Kozal JS
AD  - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA.
AD  - Integrated Toxicology and Environmental Health Program, Duke University, Durham, 
      NC, 27708, USA.
FAU - Leuthner, T C
AU  - Leuthner TC
AD  - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA.
AD  - Integrated Toxicology and Environmental Health Program, Duke University, Durham, 
      NC, 27708, USA.
FAU - Di Giulio, R T
AU  - Di Giulio RT
AD  - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA.
AD  - Integrated Toxicology and Environmental Health Program, Duke University, Durham, 
      NC, 27708, USA.
LA  - eng
GR  - P42 ES010356/ES/NIEHS NIH HHS/United States
GR  - T32 ES021432/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20170217
PL  - United States
TA  - Ecotoxicology
JT  - Ecotoxicology (London, England)
JID - 9885956
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Adaptation, Physiological
MH  - Animals
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Embryo, Nonmammalian/drug effects
MH  - Energy Metabolism
MH  - Fundulidae/*physiology
MH  - Polycyclic Aromatic Hydrocarbons/*toxicity
MH  - Water Pollutants, Chemical/*toxicity
PMC - PMC5398948
MID - NIHMS853741
OTO - NOTNLM
OT  - Atlantic killifish
OT  - Bioenergetics
OT  - CYP1A
OT  - Developmental toxicity
OT  - Polycyclic aromatic hydrocarbons
COIS- Compliance with Ethical Standards: Conflict of Interest: The authors declare that 
      they have no conflict of interest.
EDAT- 2017/02/19 06:00
MHDA- 2017/07/21 06:00
PMCR- 2018/04/01
CRDT- 2017/02/19 06:00
PHST- 2017/02/02 00:00 [accepted]
PHST- 2017/02/19 06:00 [pubmed]
PHST- 2017/07/21 06:00 [medline]
PHST- 2017/02/19 06:00 [entrez]
PHST- 2018/04/01 00:00 [pmc-release]
AID - 10.1007/s10646-017-1775-6 [pii]
AID - 10.1007/s10646-017-1775-6 [doi]
PST - ppublish
SO  - Ecotoxicology. 2017 Apr;26(3):435-448. doi: 10.1007/s10646-017-1775-6. Epub 2017 
      Feb 17.