PMID- 28214198
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20220410
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 213
IP  - 3
DP  - 2017 Mar
TI  - Tumor-derived CD4+CD25+regulatory T cells inhibit dendritic cells function by 
      CTLA-4.
PG  - 245-249
LID - S0344-0338(16)30236-9 [pii]
LID - 10.1016/j.prp.2016.12.008 [doi]
AB  - PURPOSE: CD4+CD25+regulatoryT cells (Tregs) play an important role in anti-tumor 
      immune responses. Poor prognosis and declining survival rates have intimate 
      connection with high Treg expression in cancer patients. Cytotoxic T 
      Lymphocyte-associated protein (CTLA-4) is one of the most prominent molecules on 
      Treg. In our previous research, we have demonstrated that HCC-derived Tregs can 
      interfere with Dendritic cells (DCs) function and down-modulate CD80/CD86 on DCs 
      in vitro in a cell-contact dependent way. However the mechanism of how 
      HCC-derived Treg affect DC phenotype are not very clear. Therefore, we 
      investigated the function of CTLA-4 in anti-tumor immune responses. MATERIALS AND 
      METHODS: We established BABL/C mouse with hepatocellular carcinoma model, and 
      tumor-derived Tregs were purified by magnetic cell sorting using mouse 
      CD4+CD25+regulatoryT cell isolation kit. Splenic DCs were enriched using 
      CD11c-conjugated microbeads. Then splenic DCs co-cultured with tumor-derived 
      Tregs and antibody-blocking experiments was performed. RESULTS: In our research, 
      we found the down-modulation of CD80/CD86 on DCs was inhibited by blocking 
      CTLA-4. HCC-derived Tregs down-modulated CD80/CD86 on DCs in a CTLA-4-dependent 
      way. Blockade of CTLA-4 can lead to increase DC-mediated immunity. CONCLUSION: 
      CTLA-4 play a vital role in Treg-mediated immnue inhibition and this discovery 
      can open up new ideas for the development of therapeutic strategies.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Chen, Xin
AU  - Chen X
AD  - Department of Gastroenterology and Hepatology, First Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China.
FAU - Du, Yong
AU  - Du Y
AD  - Department of Pediatrics, First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, 325000, ZheJiang, China.
FAU - Hu, Qingqing
AU  - Hu Q
AD  - Department of Pediatrics, First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, 325000, ZheJiang, China.
FAU - Huang, ZhiMing
AU  - Huang Z
AD  - Department of Gastroenterology and Hepatology, First Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China. Electronic address: 
      515359290@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20161221
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (CTLA-4 Antigen)
SB  - IM
MH  - Animals
MH  - CTLA-4 Antigen/*metabolism
MH  - Carcinoma, Hepatocellular/immunology/metabolism/pathology
MH  - Dendritic Cells/*metabolism
MH  - Liver Neoplasms/immunology/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes, Regulatory/*metabolism
OTO - NOTNLM
OT  - CD4+CD25+regulatory T cell
OT  - CTLA-4
OT  - Dendritic cell
EDAT- 2017/02/19 06:00
MHDA- 2017/04/07 06:00
CRDT- 2017/02/19 06:00
PHST- 2016/07/06 00:00 [received]
PHST- 2016/10/16 00:00 [revised]
PHST- 2016/12/15 00:00 [accepted]
PHST- 2017/02/19 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
PHST- 2017/02/19 06:00 [entrez]
AID - S0344-0338(16)30236-9 [pii]
AID - 10.1016/j.prp.2016.12.008 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2017 Mar;213(3):245-249. doi: 10.1016/j.prp.2016.12.008. Epub 
      2016 Dec 21.