PMID- 28214429
OWN - NLM
STAT- MEDLINE
DCOM- 20170918
LR  - 20220318
IS  - 1873-3344 (Electronic)
IS  - 0162-0134 (Linking)
VI  - 170
DP  - 2017 May
TI  - Interplay between autophagy and apoptosis in selenium deficient cardiomyocytes in 
      chicken.
PG  - 17-25
LID - S0162-0134(17)30078-8 [pii]
LID - 10.1016/j.jinorgbio.2017.02.006 [doi]
AB  - Dietary selenium (Se) deficiency can cause heart dysfunction, however the exact 
      mechanism remains unclear. To understand this mechanism, 180day-old chicks, 
      divided into two groups, C (control group) and L (low Se group), were fed with 
      either a Se-sufficient (0.23mg/kg) or Se-deficient (0.033mg/kg) diets for 25days, 
      respectively. Heart tissues and blood samples were collected. In L group, the 
      activities of serum creatine kinase (CK) and creatine kinase-myoglobin (CK-MB) 
      increased and typical ultrastructural apoptotic features were observed. Se 
      deficiency up-regulated the mRNA levels of Cysteinyl aspartate specific 
      proteinase 3 (Caspase-3), Cysteinyl aspartate specific proteinase 8 (Caspase-8), 
      Cysteinyl aspartate specific proteinase 9 (Caspase-9), B cell lymphoma/leukemia 2 
      (Bcl-2), Bcl-2 Associated X Protein (Bax), (P<0.05), whereas, the mRNA levels of 
      Microtubuleassociated protein light chains 3-1 (LC3-1), Autophagy associated gene 
      5 (ATG-5), Mammalian target of rapamycin (mTOR), Dynein and Becline-1 were 
      down-regulated (P<0.05). Noticeably, Microtubuleassociated protein light chains 
      3-2 (LC3-2) mRNA level increased (P<0.05) by 20%. Western blot results showed 
      that Se deficiency decreased the expression of Becline-1 and LC3-1 protein, 
      however, the expression of Bax, Caspase-3 and Cysteinyl aspartate specific 
      proteinase 12 (Caspase-12) increased at protein levels. The present study 
      revealed that Se deficiency induced apoptosis while inhibited autophagy in 
      chicken cardiomyocytes through Bax/Bcl-2 inhibition and caspases-mediated 
      cleavage of Becline-1. Moreover, correlation analysis illustrates that apoptosis 
      and autophagy might function contradictorily. Altogether we conclude that Se 
      deficient chicken cardiomyocytes experienced apoptosis rather than autophagy 
      which is considered to be more pro-survival.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Yang, Jie
AU  - Yang J
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Zhang, Yuan
AU  - Zhang Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Hamid, Sattar
AU  - Hamid S
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Cai, Jingzeng
AU  - Cai J
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Liu, Qi
AU  - Liu Q
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Li, Hao
AU  - Li H
AD  - Rizhao City Animal Husbandry and Veterinary Bureau of Juxian, Shandong Province, 
      China.
FAU - Zhao, Rihong
AU  - Zhao R
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Wang, Hong
AU  - Wang H
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Xu, Shiwen
AU  - Xu S
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China.
FAU - Zhang, Ziwei
AU  - Zhang Z
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      PR China. Electronic address: zhangziwei@neau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170210
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Avian Proteins)
RN  - 0 (Muscle Proteins)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - *Autophagy
MH  - Avian Proteins/metabolism
MH  - Chickens/*metabolism
MH  - Muscle Proteins/metabolism
MH  - Myocytes, Cardiac/*metabolism
MH  - Selenium/*deficiency
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Chickens
OT  - Heart
OT  - Selenium deficiency
EDAT- 2017/02/19 06:00
MHDA- 2017/09/19 06:00
CRDT- 2017/02/19 06:00
PHST- 2016/07/12 00:00 [received]
PHST- 2017/01/21 00:00 [revised]
PHST- 2017/02/09 00:00 [accepted]
PHST- 2017/02/19 06:00 [pubmed]
PHST- 2017/09/19 06:00 [medline]
PHST- 2017/02/19 06:00 [entrez]
AID - S0162-0134(17)30078-8 [pii]
AID - 10.1016/j.jinorgbio.2017.02.006 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2017 May;170:17-25. doi: 10.1016/j.jinorgbio.2017.02.006. Epub 
      2017 Feb 10.