PMID- 28216954
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240324
IS  - 0976-4879 (Print)
IS  - 0975-7406 (Electronic)
IS  - 0975-7406 (Linking)
VI  - 8
IP  - 4
DP  - 2016 Oct-Dec
TI  - Optimization, characterization, and efficacy evaluation of 2% chitosan scaffold 
      for tissue engineering and wound healing.
PG  - 300-308
LID - 10.4103/0975-7406.199346 [doi]
AB  - OBJECTIVE: To develop a chitosan-based scaffold and carry out a complete 
      comprehensive study encompassing optimization of exact chitosan strength, product 
      characterization, toxicity evaluation, in vitro validation in cell culture 
      experiments, and finally in vivo efficacy in animal excision wound model. 
      MATERIALS AND METHODS: Developed chitosan scaffolds (CSs) were optimized for 
      tissue engineering and wound healing efficacy by means of microstructure, 
      toxicity, and biocompatibility evaluation. RESULTS: Scanning electron microscope 
      (SEM) studies revealed that porosity of CS decreased with increase in chitosan 
      concentration. Chemical stability and integrity of scaffolds were confirmed by 
      Fourier transform infrared studies. Highest swelling percentage (SP) of 500% was 
      observed in 2%, while lowest (200%) was observed in 1% CS. Reabsorption and 
      noncytotoxic property of optimized scaffold were established by enzymatic 
      degradation and MTT assay. Enzymatic degradation suggested 20-45% of weight loss 
      (WL) within 14 days of incubation. Cytotoxicity analysis showed that scaffolds 
      were noncytotoxic against normal human dermal fibroblast human dermal fibroblast 
      cell lines. Significant cellular adherence over the scaffold surface with normal 
      cellular morphology was confirmed using SEM analysis. In vivo efficacy evaluation 
      was carried out by means of reduction in wound size on Sprague-Dawley rats. 
      Sprague-Dawley rats treated with optimized scaffold showed ~ 100% wound healing 
      in comparison to ~80% healing in betadine-treated animals within 14 days. 
      Histological examination depicted advance re-epithelization with better 
      organization of collagen bundle in wound area treated with 2% CS in comparison to 
      conventional treatment or no treatment. CONCLUSION: This study, thus, reveals 
      that 2% CSs were found to have a great potential in wound healing.
FAU - Chhabra, Priyanka
AU  - Chhabra P
AD  - Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied 
      Sciences, Defence Research and Development Organisation, New Delhi, India.
FAU - Tyagi, Priyanka
AU  - Tyagi P
AD  - Centre for Biomedical Engineering, Indian Institute of Technology, New Delhi, 
      India.
FAU - Bhatnagar, Aseem
AU  - Bhatnagar A
AD  - Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied 
      Sciences, Defence Research and Development Organisation, New Delhi, India.
FAU - Mittal, Gaurav
AU  - Mittal G
AD  - Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied 
      Sciences, Defence Research and Development Organisation, New Delhi, India.
FAU - Kumar, Amit
AU  - Kumar A
AD  - Department of Nuclear Medicine, Institute of Nuclear Medicine and Allied 
      Sciences, Defence Research and Development Organisation, New Delhi, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Pharm Bioallied Sci
JT  - Journal of pharmacy & bioallied sciences
JID - 101537209
PMC - PMC5314829
OTO - NOTNLM
OT  - Chitosan
OT  - histopathology
OT  - scaffold
OT  - tissue engineering
OT  - wound healing
COIS- There are no conflicts of interest.
EDAT- 2017/02/22 06:00
MHDA- 2017/02/22 06:01
PMCR- 2016/10/01
CRDT- 2017/02/21 06:00
PHST- 2017/02/21 06:00 [entrez]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/02/22 06:01 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - JPBS-8-300 [pii]
AID - 10.4103/0975-7406.199346 [doi]
PST - ppublish
SO  - J Pharm Bioallied Sci. 2016 Oct-Dec;8(4):300-308. doi: 10.4103/0975-7406.199346.