PMID- 28218436
OWN - NLM
STAT- MEDLINE
DCOM- 20180314
LR  - 20221207
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 43
IP  - 3
DP  - 2017 May 6
TI  - Differentially expressed haptoglobin as a potential biomarker for type 2 diabetic 
      mellitus in Hispanic population.
PG  - 424-433
LID - 10.1002/biof.1352 [doi]
AB  - Glycosylated hemoglobin (HbA1c) measurement is currently a primary tool for 
      diagnosis of type 2 diabetes mellitus (T2DM), especially for the assessment of 
      chronic hyperglycemia. However, many studies reported the limitation of using 
      HbA1c for T2DM diagnosis/prognosis, such as poor sensitivities, difficult 
      standardization, and variable cut points across ethnic groups. Therefore, the aim 
      of this study was to discover novel biomarkers associated with elevated HbA1c 
      levels as complementary T2DM diagnostic tools. Two-dimensional difference gel 
      electrophoresis combined with mass spectrometry were applied for protein profile 
      analyses of two pooled serum samples collected from Hispanic T2DM subjects 
      (n = 74) with HbA1c >/=7 and HbA1c< 7, respectively. Isoforms of haptoglobin (Hp) 
      alpha1/alpha2 chains were significantly altered in pooled serum samples from T2DM 
      subjects with HbA1c >/=7 compared to those with HbA1c< 7. Hp genotypes of 262 
      Hispanic subjects, including 109 T2DM and 153 nondiabetic controls, were further 
      determined by PCRs and western blotting analysis. Meanwhile, a new droplet 
      digital PCR method for Hp genotyping was also established. The distribution of 
      Hp2 allele was higher in T2DM subjects compared to nondiabetic controls and the 
      HbA1c levels of T2DM subjects carrying at least one Hp2 allele tended to be 
      higher than T2DM subjects with Hp 1-1. In summary, our results indicate that 
      differentially expressed serum Hp protein isoforms could be associated with HbA1c 
      levels and subjects with Hp2 allele have a higher risk for the occurrence of T2DM 
      in Hispanic population. (c) 2016 BioFactors, 43(3):424-433, 2017.
CI  - (c) 2017 International Union of Biochemistry and Molecular Biology.
FAU - Liu, Zhongwei
AU  - Liu Z
AD  - Department of Environmental Toxicology, The Institute of Environmental and Human 
      Health, Texas Tech University, Lubbock, TX.
FAU - Feng, Du
AU  - Feng D
AD  - School of Nursing, University of Nevada, Las Vegas, NV.
FAU - Gu, Danshan
AU  - Gu D
AD  - Huafang College, Xuzhou Medical University, Xuzhou, China.
FAU - Zheng, Richard
AU  - Zheng R
AD  - Department of Biology, Texas Tech University, Lubbock, TX.
FAU - Esperat, Christina
AU  - Esperat C
AD  - School of Nursing, Texas Tech University Health Sciences Center, Lubbock, TX.
FAU - Gao, Weimin
AU  - Gao W
AD  - Department of Environmental Toxicology, The Institute of Environmental and Human 
      Health, Texas Tech University, Lubbock, TX.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Biomarkers)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (HP protein, human)
RN  - 0 (Haptoglobins)
RN  - 0 (hemoglobin A1c protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Biomarkers/blood
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*blood/*diagnosis/ethnology/genetics
MH  - Female
MH  - Gene Frequency
MH  - *Genotype
MH  - Glycated Hemoglobin/*genetics/metabolism
MH  - Haptoglobins/*genetics/metabolism
MH  - Hispanic or Latino
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Proteomics/instrumentation/methods
MH  - Risk Factors
MH  - Sequence Alignment
OTO - NOTNLM
OT  - HbA1c
OT  - Hispanic
OT  - droplet digital PCR
OT  - haptoglobin
OT  - type 2 diabetes mellitus
EDAT- 2017/02/22 06:00
MHDA- 2018/03/15 06:00
CRDT- 2017/02/21 06:00
PHST- 2016/11/17 00:00 [received]
PHST- 2017/01/06 00:00 [revised]
PHST- 2017/01/17 00:00 [accepted]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2018/03/15 06:00 [medline]
PHST- 2017/02/21 06:00 [entrez]
AID - 10.1002/biof.1352 [doi]
PST - ppublish
SO  - Biofactors. 2017 May 6;43(3):424-433. doi: 10.1002/biof.1352. Epub 2017 Feb 20.