PMID- 28218690
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20181207
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 22
IP  - 2
DP  - 2017 Feb 17
TI  - Comparison of the Anti-Inflammatory Activities of Supercritical Carbon Dioxide 
      versus Ethanol Extracts from Leaves of Perilla frutescens Britt. Radiation 
      Mutant.
LID - 10.3390/molecules22020311 [doi]
LID - 311
AB  - In this study, we aimed to compare supercritical carbon dioxide extraction and 
      ethanol extraction for isoegomaketone (IK) content in perilla leaf extracts and 
      to identify the optimal method. We measured the IK concentration using HPLC and 
      inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells 
      from the extracts. The IK concentration was 10-fold higher in perilla leaf 
      extracts by supercritical carbon dioxide extraction (SFE) compared with that in 
      perilla leaf extracts by ethanol extraction (EE). When the extracts were treated 
      in LPS-induced RAW 264.7 cells at 25 mug/mL, the SFE inhibited the expression of 
      inflammatory mediators such as nitric oxide (NO), monocyte chemoattractant 
      protein-1 (MCP-1), interleutkin-6 (IL-6), interferon-beta (IFN-beta), and inducible 
      nitric oxide synthase (iNOS) to a much greater extent compared with EE. Taken 
      together, supercritical carbon dioxide extraction is considered the optimal 
      process for obtaining high IK content and anti-inflammatory activities in leaf 
      extracts from the P. frutescens Britt. radiation mutant.
FAU - Jin, Chang Hyun
AU  - Jin CH
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup-si, Jeollabuk-do 56212, Korea. snhan@snu.ac.kr.
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea. snhan@snu.ac.kr.
FAU - Park, Han Chul
AU  - Park HC
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup-si, Jeollabuk-do 56212, Korea. phc3634@kaeri.re.kr.
FAU - So, Yangkang
AU  - So Y
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup-si, Jeollabuk-do 56212, Korea. yangkang@kaeri.re.kr.
FAU - Nam, Bomi
AU  - Nam B
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup-si, Jeollabuk-do 56212, Korea. bomi1201@kaeri.re.kr.
FAU - Han, Sung Nim
AU  - Han SN
AD  - Department of Food and Nutrition, College of Human Ecology, Seoul National 
      University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea. snhan@snu.ac.kr.
FAU - Kim, Jin-Baek
AU  - Kim JB
AD  - Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 
      Jeongeup-si, Jeollabuk-do 56212, Korea. jbkim74@kaeri.re.kr.
LA  - eng
PT  - Journal Article
DEP - 20170217
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Carbon Dioxide/chemistry
MH  - Cell Survival/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Cytokines/metabolism
MH  - Ethanol/chemistry
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/drug effects/immunology/metabolism
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Perilla frutescens/*chemistry
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plant Leaves/*chemistry
PMC - PMC6155756
OTO - NOTNLM
OT  - Perilla frutescens
OT  - ethanol
OT  - extraction
OT  - isoegomaketone
OT  - radiation mutant
OT  - supercritical carbon dioxide
COIS- The authors declare no conflict of interest.
EDAT- 2017/02/22 06:00
MHDA- 2017/05/10 06:00
PMCR- 2017/02/17
CRDT- 2017/02/21 06:00
PHST- 2017/01/05 00:00 [received]
PHST- 2017/02/14 00:00 [accepted]
PHST- 2017/02/21 06:00 [entrez]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2017/02/17 00:00 [pmc-release]
AID - molecules22020311 [pii]
AID - molecules-22-00311 [pii]
AID - 10.3390/molecules22020311 [doi]
PST - epublish
SO  - Molecules. 2017 Feb 17;22(2):311. doi: 10.3390/molecules22020311.