PMID- 28218782
OWN - NLM
STAT- MEDLINE
DCOM- 20170301
LR  - 20211204
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Feb 16
TI  - Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer.
LID - 10.4238/gmr16019272 [doi]
AB  - Kruppel-like factors (KLFs) are a group of transcriptional regulators that have 
      recently been identified to exhibit tumor-suppressive function against various 
      gastrointestinal cancers. The present study aims to investigate the expression 
      patterns and prognostic value of KLF-4 in colorectal cancers (CRCs). KLF-4 levels 
      in CRC tissues were examined via immunohistochemistry analysis, real-time 
      quantitative polymerase chain reaction, and western blotting. The chi-square test 
      was performed to evaluate the correlation between KLF-4 expression and the 
      clinicopathological characteristics. Kaplan-Meier analysis was performed to 
      assess the prognostic value of KLF-4 in CRC patients. In addition, we evaluated 
      the effect of KLF-4 knockdown on the proliferation of CRC HT-29 cells. Our 
      results showed significant downregulation of KLF-4 in 31 CRC samples, collected 
      from CRC patients showing more malignant characteristics such as lymphatic 
      metastasis, low tumor cell differentiation, and tumor recurrence. CRC patients in 
      the low KLF-4 group were found to have reduced overall survival and decreased 
      disease-free survival time. Moreover, HT-29 cells transfected with siRNA-KLF-4 
      showed increased proliferation compared to those transfected with control siRNA. 
      In summary, lower KLF-4 expression was correlated with malignant CRC status and 
      poor prognosis in CRC patients. Moreover, KLF-4 suppression promoted the 
      proliferation of CRC cells in vitro. These results provide novel insights into 
      the tumor suppressive role of KLF-4 in CRC.
FAU - Xiu, D H
AU  - Xiu DH
AD  - Department of Radiology, China-Japan Union Hospital of Jilin University, 
      Changchun, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Department of Endocrinology, Second Hospital of Jilin University, Changchun, 
      China.
FAU - Liu, L
AU  - Liu L
AD  - Department of Radiology, China-Japan Union Hospital of Jilin University, 
      Changchun, China.
FAU - Yang, H S
AU  - Yang HS
AD  - Department of Radiology, China-Japan Union Hospital of Jilin University, 
      Changchun, China.
FAU - Liu, G F
AU  - Liu GF
AD  - Department of Radiology, China-Japan Union Hospital of Jilin University, 
      Changchun, China jlfsliuguifeng@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170216
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (KLF4 protein, human)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (Kruppel-Like Transcription Factors)
SB  - IM
MH  - Cell Differentiation
MH  - Colorectal Neoplasms/genetics/metabolism/*pathology
MH  - *Down-Regulation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HT29 Cells
MH  - Humans
MH  - Kruppel-Like Factor 4
MH  - Kruppel-Like Transcription Factors/*genetics/*metabolism
MH  - Lymphatic Metastasis
MH  - Male
MH  - Prognosis
MH  - Survival Analysis
EDAT- 2017/02/22 06:00
MHDA- 2017/03/03 06:00
CRDT- 2017/02/21 06:00
PHST- 2017/02/21 06:00 [entrez]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
AID - gmr-16-01-gmr.16019272 [pii]
AID - 10.4238/gmr16019272 [doi]
PST - epublish
SO  - Genet Mol Res. 2017 Feb 16;16(1). doi: 10.4238/gmr16019272.