PMID- 28219599
OWN - NLM
STAT- MEDLINE
DCOM- 20170501
LR  - 20181202
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 49
IP  - 2
DP  - 2017 Mar
TI  - Aminophylline Effect on Renal Ischemia-Reperfusion Injury in Mice.
PG  - 358-365
LID - S0041-1345(16)30952-6 [pii]
LID - 10.1016/j.transproceed.2016.11.043 [doi]
AB  - BACKGROUND: Aminophylline increases the intracellular concentration of cAMP and 
      exerts an anti-inflammatory effect. The aim of this study was to investigate the 
      effect of aminophylline on renal ischemia-reperfusion (I/R) injury in mice. 
      METHODS: Thirty C57BL/6 mice were divided into 3 groups. In the sham group (group 
      S, n = 10), only right nephrectomy was performed. In the control group (group C, 
      n = 10), after right nephrectomy, the mice were subjected to 30 minutes of left 
      renal ischemia. In the aminophylline group (group A, n = 10), an intraperitoneal 
      injection of aminophylline (5 mg/kg) was performed before renal ischemia. 
      Twenty-four hours after reperfusion, the mice were euthanized, and plasma and 
      kidney samples were obtained to analyze the serum creatinine, renal histology, 
      and expression levels of nuclear factor-kappa B (NF-kB) and pro-inflammatory 
      cytokines. RESULTS: The serum creatinine concentration in group C was markedly 
      elevated at 24 hours after reperfusion. Aminophylline treatment significantly 
      reduced serum creatinine, compared with group C. Aminophylline also reduced the 
      histological evidence of renal damage. The expression levels of NF-kB, tumor 
      necrosis factor-alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1), macrophage 
      inflammatory protein-2 (MIP-2), and intercellular adhesion molecule-1 (ICAM-1) 
      mRNA were significantly increased in group C (P < .001). Group A showed lower 
      expression of NF-kB, TNF-alpha, MCP-1, MIP-2, and ICAM-1 mRNA than group C (P < .01). 
      CONCLUSIONS: Aminophylline treatment improved the renal function and indexes of 
      renal inflammation, which suggests that it provided reno-protection against renal 
      I/R injury.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Seo, K
AU  - Seo K
AD  - Department of Anesthesiology and Pain Medicine, St Vincent's Hospital, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Choi, J W
AU  - Choi JW
AD  - Department of Anesthesiology and Pain Medicine, St Vincent's Hospital, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Kim, D-W
AU  - Kim DW
AD  - Department of Anesthesiology and Pain Medicine, St Vincent's Hospital, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Han, D
AU  - Han D
AD  - Department of Anesthesiology and Pain Medicine, St Vincent's Hospital, The 
      Catholic University of Korea, Seoul, Korea.
FAU - Noh, S J
AU  - Noh SJ
AD  - The Research Institute of Medical Science, St Vincent's Hospital, The Catholic 
      University of Korea, Seoul, Korea.
FAU - Jung, H S
AU  - Jung HS
AD  - Department of Anesthesiology and Pain Medicine, St Vincent's Hospital, The 
      Catholic University of Korea, Seoul, Korea. Electronic address: flood1@naver.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Purinergic P1 Receptor Antagonists)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 27Y3KJK423 (Aminophylline)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Acute Kidney Injury/*prevention & control
MH  - Aminophylline/*pharmacology
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Creatinine/metabolism
MH  - Cytokines/metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Kidney/blood supply
MH  - Kidney Function Tests
MH  - Male
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - Nephritis/prevention & control
MH  - Neutrophil Infiltration/drug effects
MH  - Purinergic P1 Receptor Antagonists/*pharmacology
MH  - Random Allocation
MH  - Reperfusion Injury/*prevention & control
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2017/02/22 06:00
MHDA- 2017/05/02 06:00
CRDT- 2017/02/22 06:00
PHST- 2016/06/28 00:00 [received]
PHST- 2016/10/01 00:00 [revised]
PHST- 2016/11/16 00:00 [accepted]
PHST- 2017/02/22 06:00 [entrez]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/05/02 06:00 [medline]
AID - S0041-1345(16)30952-6 [pii]
AID - 10.1016/j.transproceed.2016.11.043 [doi]
PST - ppublish
SO  - Transplant Proc. 2017 Mar;49(2):358-365. doi: 10.1016/j.transproceed.2016.11.043.