PMID- 28219760 OWN - NLM STAT- MEDLINE DCOM- 20180220 LR - 20180421 IS - 1873-1708 (Electronic) IS - 0890-6238 (Linking) VI - 69 DP - 2017 Apr TI - Maternal exposure to perfluorooctanoic acid inhibits luteal function via oxidative stress and apoptosis in pregnant mice. PG - 159-166 LID - S0890-6238(17)30090-4 [pii] LID - 10.1016/j.reprotox.2017.02.010 [doi] AB - Perfluorooctanoic acid (PFOA) is a synthetic perfluorinated compound, which has been reported to exert adverse effect on the pregnancy. However, whether it is associated with alteration of luteal function remains unknown. Mice were administered PFOA by gavage from gestational days (GD) 1-7 or 13. PFOA treatment did not significantly affect numbers of embryo implantation. Nevertheless, on GD 13, 10mg/kg PFOA treatment significantly increased numbers of resorbed embryo. Furthermore, PFOA exposure markedly reduced serum progesterone levels but did not affect estradiol levels. Treatment also showed concomitant decreases in transcript levels for key steroidogenic enzymes, and reduced numbers and sizes of corpora lutea. In addition, PFOA administration inhibited activities of superoxide dismutase and catalase, and increased generation of hydrogen peroxide and malondialdehyde, and down-regulated level of Bcl-2 and up-regulated p53 and BAX proteins. In conclusion, PFOA exposure significantly inhibits luteal function via oxidative stress and apoptosis in pregnant mice. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Chen, Yilu AU - Chen Y AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: yilua_chen@163.com. FAU - Zhou, Ling AU - Zhou L AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: noradrenalin@126.com. FAU - Xu, Jingjie AU - Xu J AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: xjj0605@163.com. FAU - Zhang, Lu AU - Zhang L AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 13393733537@163.com. FAU - Li, Mo AU - Li M AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 544182657@qq.com. FAU - Xie, Xingxing AU - Xie X AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 1120434253@qq.com. FAU - Xie, Yajuan AU - Xie Y AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: 1454503826@qq.com. FAU - Luo, Dan AU - Luo D AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: thinker_20080502@126.com. FAU - Zhang, Dalei AU - Zhang D AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: zhangdalei@ncu.edu.cn. FAU - Yu, Xiaochun AU - Yu X AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: physiology9909@163.com. FAU - Yang, Bei AU - Yang B AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: yangbei@ncu.edu.cn. FAU - Kuang, Haibin AU - Kuang H AD - Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China. Electronic address: kuanghaibin@ncu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170220 PL - United States TA - Reprod Toxicol JT - Reproductive toxicology (Elmsford, N.Y.) JID - 8803591 RN - 0 (3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase) RN - 0 (Caprylates) RN - 0 (Fluorocarbons) RN - 0 (Multienzyme Complexes) RN - 0 (Phosphoproteins) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 0 (Tumor Suppressor Protein p53) RN - 0 (steroidogenic acute regulatory protein) RN - 4G7DS2Q64Y (Progesterone) RN - 4TI98Z838E (Estradiol) RN - 4Y8F71G49Q (Malondialdehyde) RN - 947VD76D3L (perfluorooctanoic acid) RN - BBX060AN9V (Hydrogen Peroxide) RN - EC 1.1.1.145 (Progesterone Reductase) RN - EC 1.11.1.6 (Catalase) RN - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 5.3.3.- (Steroid Isomerases) SB - IM MH - Animals MH - Apoptosis/drug effects MH - Caprylates/*toxicity MH - Catalase/metabolism MH - Cholesterol Side-Chain Cleavage Enzyme/genetics MH - Embryo Implantation/drug effects MH - Estradiol/blood MH - Female MH - Fluorocarbons/*toxicity MH - Hydrogen Peroxide/metabolism MH - Malondialdehyde/metabolism MH - Mice MH - Multienzyme Complexes/genetics MH - Ovary/*drug effects/metabolism MH - Oxidative Stress/drug effects MH - Phosphoproteins/genetics MH - Pregnancy MH - Progesterone/blood MH - Progesterone Reductase/genetics MH - Proto-Oncogene Proteins c-bcl-2/metabolism MH - Steroid Isomerases/genetics MH - Superoxide Dismutase/metabolism MH - Tumor Suppressor Protein p53/metabolism OTO - NOTNLM OT - Apoptosis OT - Luteal function OT - Oxidative stress OT - Perfluorooctanoic acid OT - Reproductive toxicity EDAT- 2017/02/22 06:00 MHDA- 2018/02/21 06:00 CRDT- 2017/02/22 06:00 PHST- 2016/09/15 00:00 [received] PHST- 2017/02/09 00:00 [revised] PHST- 2017/02/15 00:00 [accepted] PHST- 2017/02/22 06:00 [pubmed] PHST- 2018/02/21 06:00 [medline] PHST- 2017/02/22 06:00 [entrez] AID - S0890-6238(17)30090-4 [pii] AID - 10.1016/j.reprotox.2017.02.010 [doi] PST - ppublish SO - Reprod Toxicol. 2017 Apr;69:159-166. doi: 10.1016/j.reprotox.2017.02.010. Epub 2017 Feb 20.