PMID- 28220308
OWN - NLM
STAT- MEDLINE
DCOM- 20170531
LR  - 20181202
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 274
IP  - 5
DP  - 2017 May
TI  - Aberrant expression of CDK8 regulates the malignant phenotype and associated with 
      poor prognosis in human laryngeal squamous cell carcinoma.
PG  - 2205-2213
LID - 10.1007/s00405-017-4484-0 [doi]
AB  - CDK8, a member of the transcriptional subtype of the cyclin-dependent kinases 
      (CDKs) family, shows remarkable cancer tissue specific expression profile and 
      rather more selective contribution to the regulation of gene expression levels 
      involved in some signaling pathways. However, the effect of CDK8 on the malignant 
      phenotype of human laryngeal squamous cell carcinoma (LSCC) cells and the 
      potential molecular mechanisms remain unclear. In the present study, we evaluated 
      the expression levels of CDK8 by quantitative real-time reverse-transcriptase 
      polymerase chain reaction (qRT-PCR) and immunohistochemistry in tissue samples of 
      60 LSCC patients. Then we analyzed and correlated the results with 
      clinicopathological features. We demonstrated that CDK8 was significantly 
      overexpressed in LSCC tissues compared with normal controls, and this 
      overexpression was correlated with lymph node metastasis and advanced clinical 
      stages. Kaplan-Meier analysis showed that high expression levels of CDK8 miRNA 
      significantly correlated with short OS survival. In addition, down-regulation of 
      CDK8 using small interfering RNA(siRNA) reduced the proliferation and migration 
      of LSCC in vitro. To explore the potential mechanism, we investigated the effect 
      of CDK8 on Wnt signaling pathway and found that CDK8 was involved in the EMT 
      progress by regulating beta-catenin of the Wnt signaling. In summary, our data 
      suggest for the first time that CDK8 appears to contribute to the malignant 
      mechanism of LSCC and may represent a significant prognostic marker for LSCC 
      patients.
FAU - Li, MingHua
AU  - Li M
AD  - Services of Head and Neck Surgery, Department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148, 
      Bao jian Road, Harbin, 150081, People's Republic of China.
FAU - Zhao, XiaoDan
AU  - Zhao X
AD  - Department of Laboratory Diagnosis, The Second Affiliated Hospital of Harbin 
      Medical University, Harbin, People's Republic of China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Services of Head and Neck Surgery, Department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148, 
      Bao jian Road, Harbin, 150081, People's Republic of China.
FAU - An, Jun
AU  - An J
AD  - Services of Head and Neck Surgery, Department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148, 
      Bao jian Road, Harbin, 150081, People's Republic of China.
FAU - Xiao, Hui
AU  - Xiao H
AD  - Services of Head and Neck Surgery, Department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148, 
      Bao jian Road, Harbin, 150081, People's Republic of China. ybbxuz2001@163.com.
FAU - Wang, Chao
AU  - Wang C
AD  - Services of Head and Neck Surgery, Department of Otolaryngology-Head and Neck 
      Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 148, 
      Bao jian Road, Harbin, 150081, People's Republic of China. hljwangchao@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 8)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/mortality
MH  - Cyclin-Dependent Kinase 8/genetics/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Neoplasms/genetics/*metabolism/mortality
MH  - Lymphatic Metastasis
MH  - Male
MH  - MicroRNAs
MH  - Middle Aged
MH  - Phenotype
MH  - Prognosis
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - CDK8
OT  - Laryngeal squamous cell carcinoma
OT  - Migration
OT  - Proliferation
OT  - Wnt
EDAT- 2017/02/22 06:00
MHDA- 2017/06/01 06:00
CRDT- 2017/02/22 06:00
PHST- 2016/12/28 00:00 [received]
PHST- 2017/01/24 00:00 [accepted]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/06/01 06:00 [medline]
PHST- 2017/02/22 06:00 [entrez]
AID - 10.1007/s00405-017-4484-0 [pii]
AID - 10.1007/s00405-017-4484-0 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2017 May;274(5):2205-2213. doi: 
      10.1007/s00405-017-4484-0. Epub 2017 Feb 20.