PMID- 28223733 OWN - NLM STAT- MEDLINE DCOM- 20170807 LR - 20181113 IS - 2219-2840 (Electronic) IS - 1007-9327 (Print) IS - 1007-9327 (Linking) VI - 23 IP - 5 DP - 2017 Feb 7 TI - Impact of humic acids on the colonic microbiome in healthy volunteers. PG - 885-890 LID - 10.3748/wjg.v23.i5.885 [doi] AB - AIM: To test the effects of humic acids on innate microbial communities of the colon. METHODS: We followed the effects of oral supplementation with humic acids (Activomin((R))) on concentrations and composition of colonic microbiome in 14 healthy volunteers for 45 d. 3 x 800 mg Activomin((R)) were taken orally for 10 d followed by 3 x 400 mg for 35 d. Colonic microbiota were investigated using multicolor fluorescence in situ hybridization (FISH) of Carnoy fixated and paraffin embedded stool cylinders. Two stool samples were collected a week prior to therapy and one stool sample on days 10, 31 and 45. Forty-one FISH probes representing different bacterial groups were used. RESULTS: The sum concentration of colonic microbiota increased from 20% at day 10 to 30% by day 31 and remained stable until day 45 (32%) of humic acid supplementation (P < 0.001). The increase in the concentrations in each person was due to growth of preexisting groups. The individual microbial profile of the patients remained unchanged. Similarly, the bacterial diversity remained stable. Concentrations of 24 of the 35 substantial groups increased from 20% to 96%. Two bacterial groups detected with Bac303 (Bacteroides) and Myc657 (mycolic acid-containing Actinomycetes) FISH probes decreased (P > 0.05). The others remained unaffected. Bacterial groups with initially marginal concentrations (< 0.1 x 10(9)/mL) demonstrated no response to humic acids. The concentrations of pioneer groups of Bifidobacteriaceae, Enterobacteriaceae and Clostridium difficile increased but the observed differences were statistically not significant. CONCLUSION: Humic acids have a profound effect on healthy colonic microbiome and may be potentially interesting substances for the development of drugs that control the innate colonic microbiome. FAU - Swidsinski, Alexander AU - Swidsinski A AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Dorffel, Yvonne AU - Dorffel Y AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Loening-Baucke, Vera AU - Loening-Baucke V AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Gille, Christoph AU - Gille C AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Reisshauer, Anne AU - Reisshauer A AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Goktas, Onder AU - Goktas O AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Kruger, Monika AU - Kruger M AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Neuhaus, Jurgen AU - Neuhaus J AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. FAU - Schrodl, Wieland AU - Schrodl W AD - Alexander Swidsinski, Vera Loening-Baucke, Christoph Gille, Anne Reisshauer, Onder Goktas, Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Department of Medicine, Section of Gastroenterology, Hepatology and Endocrinology, Charite Universitatsmedizin Berlin, 10098 Berlin, Germany. LA - eng PT - Journal Article PT - Observational Study PL - United States TA - World J Gastroenterol JT - World journal of gastroenterology JID - 100883448 RN - 0 (Humic Substances) SB - IM MH - Adult MH - Colony Count, Microbial MH - Dietary Supplements MH - Female MH - Gastrointestinal Microbiome/*drug effects/genetics/*physiology MH - Healthy Volunteers MH - Humans MH - *Humic Substances MH - In Situ Hybridization, Fluorescence MH - Male MH - Middle Aged MH - Young Adult PMC - PMC5296205 OTO - NOTNLM OT - Colonic bioreactor OT - Colonic microbiota OT - Fluorescence in situ hybridization OT - Healthy volunteers OT - Humic acids OT - Oral supplementation COIS- Conflict-of-interest statement: There are no conflicts of interest to report. EDAT- 2017/02/23 06:00 MHDA- 2017/08/08 06:00 PMCR- 2017/02/07 CRDT- 2017/02/23 06:00 PHST- 2015/10/04 00:00 [received] PHST- 2016/11/13 00:00 [revised] PHST- 2017/01/03 00:00 [accepted] PHST- 2017/02/23 06:00 [entrez] PHST- 2017/02/23 06:00 [pubmed] PHST- 2017/08/08 06:00 [medline] PHST- 2017/02/07 00:00 [pmc-release] AID - 10.3748/wjg.v23.i5.885 [doi] PST - ppublish SO - World J Gastroenterol. 2017 Feb 7;23(5):885-890. doi: 10.3748/wjg.v23.i5.885.