PMID- 28223796
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20220423
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 12
DP  - 2017
TI  - Stromelysin-2 (MMP-10) facilitates clearance and moderates inflammation and cell 
      death following lung exposure to long multiwalled carbon nanotubes.
PG  - 1019-1031
LID - 10.2147/IJN.S123484 [doi]
AB  - Multiwalled carbon nanotubes (MWCNTs) are nanomaterials composed of multiple 
      layers of graphene cylinders with unique properties that make them valuable for a 
      number of industries. However, rising global production has led to concerns 
      regarding potential occupational exposures to them as raw materials during 
      handling. This is especially true for long MWCNT fibers, whose aspect ratio has 
      been posited to initiate pathology similar to that of asbestos. Matrix 
      metalloproteinases (MMPs) are a class of extracellular endopeptidases that 
      control various processes related to tissue repair, inflammation, and more. 
      Stromelysin-2 (MMP-10) has roles in modulating macrophage activation and 
      function, and hence, we used an MMP-10 null (Mmp10(-/-)) mouse model to assess 
      its role in controlling lung responses to inhaled long MWCNTs. Oropharyngeal 
      aspiration of long MWCNTs (80 microg/mouse) by wild-type mice induced expression of 
      Mmp10 mRNA, which was accompanied by a robust inflammatory response characterized 
      by elevated expression of Tnfa, Il6, and Il1b. In Mmp10(-/-) mice, we found that 
      absence of MMP-10 led to impaired pulmonary clearance of MWCNTs and reduced 
      macrophage cell survival. Exposure of wild-type bone marrow-derived macrophages 
      (BMDMs) and alveolar macrophages to MWCNTs caused a rapid, dose-dependent 
      upregulation of Mmp10 mRNA expression, which was accompanied by expression of 
      pro-inflammatory products (Il6 and Il1b). These products were further enhanced in 
      Mmp10(-/-) macrophages, resulting in increased caspase-3-dependent cell death 
      compared with wild-type cells. These findings indicate that MMP-10 facilitates 
      the clearance of MWCNTs and moderates the pro-inflammatory response of exposed 
      alveolar and infiltrated macrophages.
FAU - Vandivort, Tyler C
AU  - Vandivort TC
AD  - Cedars-Sinai Medical Center, Women's Guild Lung Institute, Los Angeles, CA; 
      Department of Environmental and Occupational Health Sciences, University of 
      Washington, Seattle, WA.
FAU - Birkland, Timothy P
AU  - Birkland TP
AD  - Cedars-Sinai Medical Center, Women's Guild Lung Institute, Los Angeles, CA.
FAU - Domiciano, Talita P
AU  - Domiciano TP
AD  - Department of Pediatrics, Cedars-Sinai Medical Center, Los Angeles, CA.
FAU - Mitra, Somenath
AU  - Mitra S
AD  - Department of Chemistry and Environmental Science, New Jersey Institute of 
      Technology, Newark, NJ, USA.
FAU - Kavanagh, Terrance J
AU  - Kavanagh TJ
AD  - Department of Environmental and Occupational Health Sciences, University of 
      Washington, Seattle, WA.
FAU - Parks, William C
AU  - Parks WC
AD  - Cedars-Sinai Medical Center, Women's Guild Lung Institute, Los Angeles, CA.
LA  - eng
GR  - R01 HL141078/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20170207
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Inflammation Mediators)
RN  - 0 (Nanotubes, Carbon)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.24.22 (Matrix Metalloproteinase 10)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage
MH  - Caspase 3/metabolism
MH  - Cell Death/drug effects
MH  - Cytoprotection/drug effects
MH  - Endocytosis/drug effects
MH  - Inflammation/*enzymology
MH  - Inflammation Mediators/metabolism
MH  - Lung/drug effects/*enzymology/*pathology
MH  - Macrophages, Alveolar/drug effects/metabolism/pathology
MH  - Matrix Metalloproteinase 10/*metabolism
MH  - Mice, Inbred C57BL
MH  - Nanotubes, Carbon/*toxicity
MH  - Pneumonia/pathology
PMC - PMC5304974
OTO - NOTNLM
OT  - MMP-10
OT  - apoptosis
OT  - lung injury
OT  - macrophages
OT  - multiwalled carbon nanotubes
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2017/02/23 06:00
MHDA- 2017/04/18 06:00
PMCR- 2017/02/07
CRDT- 2017/02/23 06:00
PHST- 2017/02/23 06:00 [entrez]
PHST- 2017/02/23 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2017/02/07 00:00 [pmc-release]
AID - ijn-12-1019 [pii]
AID - 10.2147/IJN.S123484 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2017 Feb 7;12:1019-1031. doi: 10.2147/IJN.S123484. 
      eCollection 2017.