PMID- 28225889
OWN - NLM
STAT- MEDLINE
DCOM- 20171113
LR  - 20181113
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 50
IP  - 3
DP  - 2017 Feb 20
TI  - Roles of monocyte chemotactic protein 1 and nuclear factor-kappaB in immune response 
      to spinal tuberculosis in a New Zealand white rabbit model.
PG  - e5625
LID - S0100-879X2017000300602 [pii]
LID - 10.1590/1414-431X20165625 [doi]
LID - e5625
AB  - This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) 
      and nuclear factor kappa B (NF-kappaB) in immune response to spinal tuberculosis in a 
      New Zealand white rabbit model. Forty-eight New Zealand white rabbits were 
      collected and divided into four groups: experimental group (n=30, spinal 
      tuberculosis model was established), the sham group (n=15, sham operation was 
      performed) and the blank group (n=3). The qRT-PCR assay and western blotting were 
      applied to detect the mRNA and protein expressions of MCP-1 and NF-kappaB in 
      peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-kappaB, IFN-gamma, 
      IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of 
      CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte subsets. Compared with the 
      sham and blank groups, the mRNA and protein expressions of MCP-1 and NF-kappaB in the 
      experimental group were significantly increased. The experimental group had lower 
      serum levels of IL-2 and IFN-gamma and higher serum level of IL-10 than the sham and 
      blank groups. In comparison to the sham and blank groups, CD4+ T lymphocyte 
      subsets percentage, CD4+/CD8+ ratio and CD4+ CD25+ Foxp3+ Tregs subsets 
      accounting for CD4+ lymphocyte in the experimental group were lower, while 
      percentage of CD8+ T lymphocyte subsets was higher. Our study provided evidence 
      that higher expression of MCP-1 and NF-kappaB may be associated with decreased immune 
      function of spinal tuberculosis, which can provide a new treatment direction for 
      spinal tuberculosis.
FAU - Guo, X H
AU  - Guo XH
AD  - The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
FAU - Bai, Z
AU  - Bai Z
AD  - The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
FAU - Qiang, B
AU  - Qiang B
AD  - The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
FAU - Bu, F H
AU  - Bu FH
AD  - Operating Room, the Fifth Hospital of Harbin, Harbin, China.
FAU - Zhao, N
AU  - Zhao N
AD  - The Third Department of Orthopedics, the Fifth Hospital of Harbin, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chemokine CCL2/blood/*metabolism
MH  - Cytokines/blood/immunology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Immunity, Cellular/*immunology
MH  - Male
MH  - NF-kappa B/blood/*metabolism
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction
MH  - Tuberculosis, Spinal/*immunology
PMC - PMC5333719
EDAT- 2017/02/23 06:00
MHDA- 2017/11/14 06:00
PMCR- 2017/02/20
CRDT- 2017/02/23 06:00
PHST- 2016/06/25 00:00 [received]
PHST- 2016/10/25 00:00 [accepted]
PHST- 2017/02/23 06:00 [entrez]
PHST- 2017/02/23 06:00 [pubmed]
PHST- 2017/11/14 06:00 [medline]
PHST- 2017/02/20 00:00 [pmc-release]
AID - S0100-879X2017000300602 [pii]
AID - 10.1590/1414-431X20165625 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2017 Feb 20;50(3):e5625. doi: 10.1590/1414-431X20165625.