PMID- 28226282 OWN - NLM STAT- MEDLINE DCOM- 20170628 LR - 20171116 IS - 1873-264X (Electronic) IS - 0731-7085 (Linking) VI - 138 DP - 2017 May 10 TI - Systematic identification of flavonols, flavonol glycosides, triterpene and siraitic acid glycosides from Siraitia grosvenorii using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening strategy. PG - 240-248 LID - S0731-7085(16)30991-8 [pii] LID - 10.1016/j.jpba.2017.01.059 [doi] AB - The fruits of Siraitia grosvenorii are considered to be health-promoting because of the diversity of their bioactive ingredients. In the present study, a screening method, using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening strategy, has been established. The technology was used to systematically screening the targeted metabolites, primarily from the complex matrix of S. grosvenorii. The compounds were then identified by their exact masses and characteristic fragment ions, in comparison with the fragmentation behaviors of 19 references. Finally, 122 compounds, including 53 flavonols and flavonol glycosides, 59 triterpene glycosides and 10 siraitic acid glycosides, were screened and identified in 10-, 50- and 80-day fruits, roots, stems and leaves of S. grosvenorii. 98 of them were reported for the first time. Additionally, the distribution of all identified components in different parts of the plant was determined and metabolic networks for flavonol and triterpene glycosides were proposed. CI - Copyright (c) 2017. Published by Elsevier B.V. FAU - Qing, Zhi-Xing AU - Qing ZX AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: 719764893@qq.com. FAU - Zhao, Huan AU - Zhao H AD - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: 53522722@163.com. FAU - Tang, Qi AU - Tang Q AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: tangqi423@sina.com. FAU - Mo, Chang-Ming AU - Mo CM AD - Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. Electronic address: mochming@163.com. FAU - Huang, Peng AU - Huang P AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: phuang1985@126.com. FAU - Cheng, Pi AU - Cheng P AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 18027474@qq.com. FAU - Yang, Peng AU - Yang P AD - College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: 137292113@qq.com. FAU - Yang, Xue-Yi AU - Yang XY AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 21811025@qq.com. FAU - Liu, Xiu-Bin AU - Liu XB AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: 87135168@qq.com. FAU - Zheng, Ya-Jie AU - Zheng YJ AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: zhengyj4@126.com. FAU - Zeng, Jian-Guo AU - Zeng JG AD - National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: zengjianguo@hunau.edu.cn. LA - eng PT - Journal Article DEP - 20170216 PL - England TA - J Pharm Biomed Anal JT - Journal of pharmaceutical and biomedical analysis JID - 8309336 RN - 0 (Flavonols) RN - 0 (Glycosides) RN - 0 (Triterpenes) SB - IM MH - Chromatography, High Pressure Liquid/*methods MH - Flavonols/*chemistry MH - Fruit/chemistry MH - Glycosides/*chemistry MH - Magnoliopsida/*chemistry MH - Mass Spectrometry/*methods MH - Triterpenes/*chemistry OTO - NOTNLM OT - Flavonol glycosides OT - HPLC-Q-TOF-MS OT - Siraitia grosvenorii OT - Siraitic acid glycosides OT - Triterpene glycosides EDAT- 2017/02/23 06:00 MHDA- 2017/06/29 06:00 CRDT- 2017/02/23 06:00 PHST- 2016/10/30 00:00 [received] PHST- 2017/01/23 00:00 [revised] PHST- 2017/01/25 00:00 [accepted] PHST- 2017/02/23 06:00 [pubmed] PHST- 2017/06/29 06:00 [medline] PHST- 2017/02/23 06:00 [entrez] AID - S0731-7085(16)30991-8 [pii] AID - 10.1016/j.jpba.2017.01.059 [doi] PST - ppublish SO - J Pharm Biomed Anal. 2017 May 10;138:240-248. doi: 10.1016/j.jpba.2017.01.059. Epub 2017 Feb 16.