PMID- 28228044
OWN - NLM
STAT- MEDLINE
DCOM- 20170309
LR  - 20201209
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Print)
IS  - 1388-0209 (Linking)
VI  - 55
IP  - 1
DP  - 2017 Dec
TI  - Withaferin A protects against spinal cord injury by inhibiting apoptosis and 
      inflammation in mice.
PG  - 1171-1176
LID - 10.1080/13880209.2017.1288262 [doi]
AB  - CONTEXT: Withaferin A (WFA) exhibits diverse pharmaceutical applications on human 
      diseases, including rheumatoid arthritis, cancers and microbial infection. 
      OBJECTIVE: We evaluated the neuroprotective role of WFA using a mouse model of 
      spinal cord injury (SCI). MATERIALS AND METHODS: BALB/c mice were administrated 
      10 mg/kg of WFA. Gene expression was measured by real-time PCR, western blot and 
      immunohistochemistry. Cell morphology and apoptosis were determined by H&E 
      staining and TUNEL assay. Motor function was evaluated by the BBB functional 
      scale for continuous 7 weeks. RESULTS: WFA significantly improved 
      neurobehavioural function and alleviated histological alteration of spinal cord 
      tissues in traumatized mice. Brain-derived neurotrophic factor (BDNF) and glial 
      cell line-derived neurotrophic factor (GDNF) significantly increased in 
      WFA-treated mice. Meanwhile, the expression of Nogo-A and RhoA remarkably 
      decreased in the presence of WFA. Furthermore, the apoptotic cell death was 
      attenuated in mice treated with WFA (31.48 +/- 2.50% vs. 50.08 +/- 2.08%) accompanied 
      by decreased bax and increased bcl-2. In addition, WFA decreased the expression 
      of pro-inflammatory mediators such as IL-1beta (11.20 +/- 1.96 ng/mL vs. 
      17.59 +/- 1.42 ng/mL) and TNF-alpha (57.38 +/- 3.57 pg/mL vs. 95.06 +/- 9.13 pg/mL). The 
      anti-inflammatory cytokines including TGF-beta1 (14.32 +/- 1.04 pg/mL vs. 
      9.37 +/- 1.17 pg/mL) and IL-10 (116.80 +/- 6.91 pg/mL vs. 72.33 +/- 9.35 pg/mL) were 
      elevated after WFA administration. DISCUSSION AND CONCLUSION: This study 
      demonstrated that WFA has a neuroprotective role by inhibition of apoptosis and 
      inflammation after SCI in mice.
FAU - Yan, Xianlei
AU  - Yan X
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Huang, Guangxiang
AU  - Huang G
AD  - b Department of Neurosurgery , The First Affiliated Hospital of Guangxi Medical 
      University , Nanning , China.
FAU - Liu, Quan
AU  - Liu Q
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Zheng, Jiemin
AU  - Zheng J
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Chen, Hongmou
AU  - Chen H
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Huang, Qidan
AU  - Huang Q
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Chen, Jiakang
AU  - Chen J
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
FAU - Huang, Heqing
AU  - Huang H
AD  - a Department of Neurosurgery , the Fourth Affiliated Hospital of Guangxi Medical 
      University , Liuzhou , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Nogo Proteins)
RN  - 0 (Rtn4 protein, mouse)
RN  - 0 (Withanolides)
RN  - EC 3.6.5.2 (RhoA protein, mouse)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - L6DO3QW4K5 (withaferin A)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Behavior, Animal/drug effects
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Disease Models, Animal
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Inflammation/metabolism/pathology/physiopathology/*prevention & control
MH  - Inflammation Mediators/metabolism
MH  - Mice, Inbred BALB C
MH  - Motor Activity/drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Nogo Proteins/metabolism
MH  - Signal Transduction/drug effects
MH  - Spinal Cord/*drug effects/metabolism/pathology/physiopathology
MH  - Spinal Cord Injuries/*drug therapy/metabolism/pathology/physiopathology
MH  - Time Factors
MH  - Withanolides/*pharmacology
MH  - rho GTP-Binding Proteins/metabolism
MH  - rhoA GTP-Binding Protein
PMC - PMC6130570
OTO - NOTNLM
OT  - Central nervous system
OT  - anti-inflammatory cytokine
OT  - natural product
OT  - pro-inflammatory cytokine
EDAT- 2017/02/24 06:00
MHDA- 2017/03/10 06:00
PMCR- 2017/02/23
CRDT- 2017/02/24 06:00
PHST- 2017/02/24 06:00 [entrez]
PHST- 2017/02/24 06:00 [pubmed]
PHST- 2017/03/10 06:00 [medline]
PHST- 2017/02/23 00:00 [pmc-release]
AID - 1288262 [pii]
AID - 10.1080/13880209.2017.1288262 [doi]
PST - ppublish
SO  - Pharm Biol. 2017 Dec;55(1):1171-1176. doi: 10.1080/13880209.2017.1288262.