PMID- 28229407
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20181202
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1553
DP  - 2017
TI  - Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells.
PG  - 55-65
LID - 10.1007/978-1-4939-6756-8_5 [doi]
AB  - Adipose tissue-derived stem cells (ADSC) are promising candidates for therapeutic 
      applications in cardiovascular regenerative medicine. By definition, the 
      phenotype ADSCs, e.g., the ubiquitous secretion of growth factors, cytokines, and 
      extracellular matrix components is not met in vivo, which renders ADSC a culture 
      "artefact." The medium constituents therefore impact the efficacy of ADSC. Little 
      attention has been paid to the energy source in medium, i.e., glucose, which 
      feeds the cell's power plants: mitochondria. The role of mitochondria in stem 
      cell biology goes beyond their function in ATP synthesis, because it includes 
      cell signaling, reactive oxygen species (ROS) production, regulation of 
      apoptosis, and aging. Appropriate application of ADSC for stem cells therapy of 
      cardiovascular disease warrants knowledge of their mitochondrial phenotype and 
      function. We discuss several methodologies for assessing ADSC mitochondrial 
      function and structural changes under environmental cues, in particular, 
      increased ROS caused by hyperglycemia.
FAU - Hajmousa, Ghazaleh
AU  - Hajmousa G
AD  - Cardiovascular Regenerative Medicine Research Group of the Department of 
      Pathology and Medical Biology, University of Groningen, University Medical Center 
      Groningen, Hanzeplein 1 - EA11, 9713 GZ, Groningen, The Netherlands.
FAU - Harmsen, Martin C
AU  - Harmsen MC
AD  - Cardiovascular Regenerative Medicine Research Group of the Department of 
      Pathology and Medical Biology, University of Groningen, University Medical Center 
      Groningen, Hanzeplein 1 - EA11, 9713 GZ, Groningen, The Netherlands. 
      m.c.harmsen@umcg.nl.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - 0 (Reactive Oxygen Species)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
EIN - Methods Mol Biol. 2017;1553:E3. PMID: 28986858
MH  - Adipose Tissue/*cytology
MH  - Apoptosis
MH  - Biomarkers
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - *Energy Metabolism
MH  - Flow Cytometry
MH  - Glucose/metabolism
MH  - Humans
MH  - Membrane Potential, Mitochondrial
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Mitochondria/metabolism
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - ADSC
OT  - Energy metabolism
OT  - Hyperglycemia
OT  - Mitochondria
OT  - ROS
EDAT- 2017/02/24 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/02/24 06:00
PHST- 2017/02/24 06:00 [entrez]
PHST- 2017/02/24 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
AID - 10.1007/978-1-4939-6756-8_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1553:55-65. doi: 10.1007/978-1-4939-6756-8_5.