PMID- 28229459
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20181221
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Print)
IS  - 0022-3751 (Linking)
VI  - 595
IP  - 11
DP  - 2017 Jun 1
TI  - Differential serotonergic modulation across the main and accessory olfactory 
      bulbs.
PG  - 3515-3533
LID - 10.1113/JP273945 [doi]
AB  - KEY POINTS: There are serotonergic projections to both the main (MOB) and the 
      accessory olfactory bulb (AOB). Current-clamp experiments demonstrate that 
      serotonergic afferents are largely excitatory for mitral cells (MCs) in the MOB 
      where 5-HT(2A) receptors mediate a direct excitatory action. Serotonergic 
      afferents are predominately inhibitory for MCs in the AOB. There are two types of 
      inhibition: indirect inhibition mediated through the 5-HT(2) receptors on 
      GABAergic interneurons and direct inhibition via the 5-HT(1) receptors on MCs. 
      Differential 5-HT neuromodulation of MCs across the MOB and AOB could contribute 
      to select behaviours such as olfactory learning or aggression. ABSTRACT: Mitral 
      cells (MCs) contained in the main (MOB) and accessory (AOB) olfactory bulb have 
      distinct intrinsic membrane properties but the extent of neuromodulation across 
      the two systems has not been widely explored. Herein, we investigated a widely 
      distributed CNS modulator, serotonin (5-HT), for its ability to modulate the 
      biophysical properties of MCs across the MOB and AOB, using an in vitro, brain 
      slice approach in postnatal 15-30 day mice. In the MOB, 5-HT elicited three types 
      of responses in 93% of 180 cells tested. Cells were either directly excited 
      (70%), inhibited (10%) or showed a mixed response (13%)- first inhibition 
      followed by excitation. In the AOB, 82% of 148 cells were inhibited with 18% of 
      cells showing no response. Albeit located in parallel partitions of the olfactory 
      system, 5-HT largely elicited MC excitation in the MOB while it evoked two 
      different kinetic rates of MC inhibition in the AOB. Using a combination of 
      pharmacological agents, we found that the MC excitatory responses in the MOB were 
      mediated by 5-HT(2A) receptors through a direct activation. In comparison, 
      5-HT-evoked inhibitory responses in the AOB arose due to a polysynaptic, 
      slow-onset inhibition attributed to 5-HT(2) receptor activation exciting 
      GABAergic interneurons. The second type of inhibition had a rapid onset as a 
      result of direct inhibition mediated by the 5-HT(1) class of receptors. The 
      distinct serotonergic modulation of MCs between the MOB and AOB could provide a 
      molecular basis for differential chemosensory behaviours driven by the brainstem 
      raphe nuclei into these parallel systems.
CI  - (c) 2017 The Authors. The Journal of Physiology (c) 2017 The Physiological Society.
FAU - Huang, Zhenbo
AU  - Huang Z
AD  - Program in Neuroscience, The Florida State University, Tallahassee, FL, USA.
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL, 
      USA.
FAU - Thiebaud, Nicolas
AU  - Thiebaud N
AD  - Program in Neuroscience, The Florida State University, Tallahassee, FL, USA.
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL, 
      USA.
FAU - Fadool, Debra Ann
AU  - Fadool DA
AUID- ORCID: 0000-0002-0546-3720
AD  - Program in Neuroscience, The Florida State University, Tallahassee, FL, USA.
AD  - Institute of Molecular Biophysics, The Florida State University, Tallahassee, FL, 
      USA.
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL, 
      USA.
LA  - eng
GR  - R01 DC013080/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170331
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Receptor, Serotonin, 5-HT2A)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Female
MH  - Interneurons/metabolism/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Olfactory Bulb/cytology/*metabolism/physiology
MH  - Receptor, Serotonin, 5-HT2A/metabolism
MH  - Sensory Receptor Cells/metabolism/physiology
MH  - Serotonergic Neurons/*metabolism/physiology
MH  - Serotonin/*metabolism
MH  - Synaptic Potentials
PMC - PMC5451723
OTO - NOTNLM
OT  - 5-HT
OT  - mitral cell
OT  - olfaction
OT  - olfactory bulb
OT  - serotonin
EDAT- 2017/02/24 06:00
MHDA- 2018/02/27 06:00
PMCR- 2018/06/01
CRDT- 2017/02/24 06:00
PHST- 2016/12/19 00:00 [received]
PHST- 2017/02/12 00:00 [accepted]
PHST- 2017/02/24 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
PHST- 2017/02/24 06:00 [entrez]
PHST- 2018/06/01 00:00 [pmc-release]
AID - TJP12295 [pii]
AID - 10.1113/JP273945 [doi]
PST - ppublish
SO  - J Physiol. 2017 Jun 1;595(11):3515-3533. doi: 10.1113/JP273945. Epub 2017 Mar 31.