PMID- 28235632
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20220311
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 66
DP  - 2017 Aug
TI  - Utility of comprehensive genomic sequencing for detecting HER2-positive 
      colorectal cancer.
PG  - 1-9
LID - S0046-8177(17)30051-5 [pii]
LID - 10.1016/j.humpath.2017.02.004 [doi]
AB  - HER2-targeted therapy is considered effective for KRAS codon 12/13 wild-type, 
      HER2-positive metastatic colorectal cancer (CRC). In general, HER2 status is 
      determined by the use of immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH). Comprehensive genomic sequencing (CGS) enables the 
      detection of gene mutations and copy number alterations including KRAS mutation 
      and HER2 amplification; however, little is known about the utility of CGS for 
      detecting HER2-positive CRC. To assess its utility, we retrospectively 
      investigated 201 patients with stage I-IV CRC. The HER2 status of the primary 
      site was assessed using IHC and FISH, and HER2 amplification of the primary site 
      was also assessed using CGS, and the findings of these approaches were compared 
      in each patient. CGS successfully detected alterations in 415 genes including 
      KRAS codon 12/13 mutation and HER2 amplification. Fifty-nine (29%) patients had a 
      KRAS codon 12/13 mutation. Ten (5%) patients were diagnosed as HER2 positive 
      because of HER2 IHC 3+, and the same 10 (5%) patients had HER2 amplification 
      evaluated using CGS. The results of HER2 status and HER2 amplification were 
      completely identical in all 201 patients (P < .001). Nine of the 10 HER2-positive 
      patients were KRAS 12/13 wild-type and were considered possible candidates for 
      HER2-targeted therapy. CGS has the same utility as IHC and FISH for detecting 
      HER2-positive patients who are candidates for HER2-targeted therapy, and 
      facilitates precision medicine and tailor-made treatment.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Shimada, Yoshifumi
AU  - Shimada Y
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan. Electronic address: 
      shimaday@med.niigata-u.ac.jp.
FAU - Yagi, Ryoma
AU  - Yagi R
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Kameyama, Hitoshi
AU  - Kameyama H
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Nagahashi, Masayuki
AU  - Nagahashi M
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Ichikawa, Hiroshi
AU  - Ichikawa H
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Tajima, Yosuke
AU  - Tajima Y
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Okamura, Takuma
AU  - Okamura T
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Nakano, Mae
AU  - Nakano M
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Nakano, Masato
AU  - Nakano M
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Sato, Yo
AU  - Sato Y
AD  - Department of Digestive and General Surgery, Uonuma Institute of Community 
      Medicine, Niigata University Medical and Dental Hospital, Niigata 949-7302, 
      Japan.
FAU - Matsuzawa, Takeaki
AU  - Matsuzawa T
AD  - Department of Digestive and General Surgery, Uonuma Institute of Community 
      Medicine, Niigata University Medical and Dental Hospital, Niigata 949-7302, 
      Japan.
FAU - Sakata, Jun
AU  - Sakata J
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Kobayashi, Takashi
AU  - Kobayashi T
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
FAU - Nogami, Hitoshi
AU  - Nogami H
AD  - Department of Surgery, Niigata Cancer Center Hospital, Niigata 951-8566, Japan.
FAU - Maruyama, Satoshi
AU  - Maruyama S
AD  - Department of Surgery, Niigata Cancer Center Hospital, Niigata 951-8566, Japan.
FAU - Takii, Yasumasa
AU  - Takii Y
AD  - Department of Surgery, Niigata Cancer Center Hospital, Niigata 951-8566, Japan.
FAU - Kawasaki, Takashi
AU  - Kawasaki T
AD  - Department of Pathology, Niigata Cancer Center Hospital, Niigata 951-8566, Japan.
FAU - Homma, Kei-Ichi
AU  - Homma KI
AD  - Department of Pathology, Niigata Cancer Center Hospital, Niigata 951-8566, Japan.
FAU - Izutsu, Hiroshi
AU  - Izutsu H
AD  - Diagnostics Research Department, Life innovation Research Institute, Denka 
      Innovation Center, Denka Co, Ltd, Tokyo 194-8560, Japan.
FAU - Kodama, Keisuke
AU  - Kodama K
AD  - Diagnostics Research Department, Life innovation Research Institute, Denka 
      Innovation Center, Denka Co, Ltd, Tokyo 194-8560, Japan.
FAU - Ring, Jennifer E
AU  - Ring JE
AD  - KEW, Inc., Cambridge, MA 02139, USA.
FAU - Protopopov, Alexei
AU  - Protopopov A
AD  - KEW, Inc., Cambridge, MA 02139, USA.
FAU - Lyle, Stephen
AU  - Lyle S
AD  - KEW, Inc., Cambridge, MA 02139, USA; University of Massachusetts Medical School, 
      Worcester, MA 01655, USA.
FAU - Okuda, Shujiro
AU  - Okuda S
AD  - Division of Bioinformatics, Niigata University Graduate School of Medical and 
      Dental Sciences, Niigata 951-8510, Japan.
FAU - Akazawa, Kohei
AU  - Akazawa K
AD  - Department of Medical Informatics, Niigata University Graduate School of Medical 
      and Dental Sciences, Niigata 951-8510, Japan.
FAU - Wakai, Toshifumi
AU  - Wakai T
AD  - Division of Digestive and General Surgery, Niigata University Graduate School of 
      Medical and Dental Sciences, Niigata 951-8510, Japan.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170222
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (KRAS protein, human)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Biopsy
MH  - Colorectal Neoplasms/chemistry/*genetics/pathology/therapy
MH  - DNA Mutational Analysis
MH  - Female
MH  - *Gene Amplification
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Phenotype
MH  - Precision Medicine
MH  - Predictive Value of Tests
MH  - Proto-Oncogene Proteins p21(ras)/genetics
MH  - Receptor, ErbB-2/analysis/*genetics
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - *Sequence Analysis, DNA
OTO - NOTNLM
OT  - Colorectal cancer
OT  - Comprehensive genomic sequencing
OT  - Fluorescence in situ hybridization
OT  - HER2
OT  - Immunohistochemistry
OT  - Next-generation sequencing
EDAT- 2017/02/27 06:00
MHDA- 2017/10/03 06:00
CRDT- 2017/02/26 06:00
PHST- 2016/11/18 00:00 [received]
PHST- 2017/01/18 00:00 [revised]
PHST- 2017/02/09 00:00 [accepted]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2017/02/26 06:00 [entrez]
AID - S0046-8177(17)30051-5 [pii]
AID - 10.1016/j.humpath.2017.02.004 [doi]
PST - ppublish
SO  - Hum Pathol. 2017 Aug;66:1-9. doi: 10.1016/j.humpath.2017.02.004. Epub 2017 Feb 
      22.