PMID- 28235637
OWN - NLM
STAT- MEDLINE
DCOM- 20170501
LR  - 20170501
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 63
DP  - 2017 Apr
TI  - Inhibition of miR-92d-3p enhances inflammation responses in genetically improved 
      farmed tilapia (GIFT, Oreochromis niloticus) with Streptococcus iniae infection 
      by modulating complement C3.
PG  - 367-375
LID - S1050-4648(17)30108-0 [pii]
LID - 10.1016/j.fsi.2017.02.036 [doi]
AB  - MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene 
      expression by binding to the 3'-untranslated regions (3'-UTRs) of their target 
      mRNAs. The miR-92 family is an important miRNA family, which was discovered to be 
      related to regulation of tumor proliferation, apoptosis, invasion, and 
      metastasis. Inhibition of miR-92d-3p was found previously in head kidney of 
      genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to 
      Streptococcus iniae infection. In this study, we found that miR-92d-3p regulated 
      complement C3 mRNA levels by binding to its 3'-UTR by 3'-UTR luciferase reporter 
      assay, and reduced miR-92d-3p expression resulted in increased C3 mRNA levels. We 
      detected a negative relationship between the expression levels of miR-92d-3p and 
      C3 in GIFT injected with miRNA antagomir. We performed in vivo functional 
      analysis by miR-92d-3p silencing. Inhibition of miR-92d-3p levels in GIFT head 
      kidney caused a significant increase in C3 expression, which consequently 
      increased the white blood cell counts and interleukin-1beta, tumor necrosis 
      factor-alpha, and interferon-gamma mRNA levels, all of which may help to activate the 
      inflammatory response in GIFT post-infection with S. iniae. Our findings indicate 
      that miR-92d-3p regulated C3 levels by binding with the C3 mRNA 3'-UTR, and this 
      interaction affected S. iniae infection induction and the immune response in 
      GIFT. We concluded that miR-92d-3p plays an important role in modulating the 
      inflammatory response in GIFT head kidney. Our findings may contribute to 
      understanding the mechanisms of miRNA-mediated gene regulation in tilapia in 
      response to S. iniae infection.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Qiang, Jun
AU  - Qiang J
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China. Electronic address: 
      Qiangj@ffrc.cn.
FAU - Tao, Yi-Fan
AU  - Tao YF
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - He, Jie
AU  - He J
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China.
FAU - Li, Hong-Xia
AU  - Li HX
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China.
FAU - Xu, Pao
AU  - Xu P
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China. Electronic address: Xup@ffrc.cn.
FAU - Bao, Jin-Wen
AU  - Bao JW
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Sun, Yi-Lan
AU  - Sun YL
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of 
      Fishery Sciences, Wuxi 214081, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20170222
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Complement C3)
RN  - 0 (Fish Proteins)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Cichlids/genetics
MH  - Complement C3/*genetics/metabolism
MH  - Fish Diseases/*genetics/immunology/microbiology
MH  - Fish Proteins/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - Immunity, Innate
MH  - Inflammation/genetics/immunology/microbiology/*veterinary
MH  - MicroRNAs/*genetics/metabolism
MH  - Streptococcal Infections/genetics/immunology/microbiology/*veterinary
MH  - Streptococcus iniae/physiology
OTO - NOTNLM
OT  - Complement C3
OT  - GIFT
OT  - Head kidney
OT  - Inflammatory response
OT  - miR-92d-3p
EDAT- 2017/02/27 06:00
MHDA- 2017/05/02 06:00
CRDT- 2017/02/26 06:00
PHST- 2016/09/12 00:00 [received]
PHST- 2016/12/27 00:00 [revised]
PHST- 2017/02/20 00:00 [accepted]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/05/02 06:00 [medline]
PHST- 2017/02/26 06:00 [entrez]
AID - S1050-4648(17)30108-0 [pii]
AID - 10.1016/j.fsi.2017.02.036 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2017 Apr;63:367-375. doi: 10.1016/j.fsi.2017.02.036. Epub 
      2017 Feb 22.