PMID- 28235682
OWN - NLM
STAT- MEDLINE
DCOM- 20170526
LR  - 20201027
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 505
DP  - 2017 May
TI  - Nuclear imprisonment of host cellular mRNA by nsp1beta protein of porcine 
      reproductive and respiratory syndrome virus.
PG  - 42-55
LID - S0042-6822(17)30036-3 [pii]
LID - 10.1016/j.virol.2017.02.004 [doi]
AB  - Positive-strand RNA genomes function as mRNA for viral protein synthesis which is 
      fully reliant on host cell translation machinery. Competing with cellular protein 
      translation apparatus needs to ensure the production of viral proteins, but this 
      also stifles host innate defense. In the present study, we showed that porcine 
      reproductive and respiratory syndrome virus (PRRSV), whose replication takes 
      place in the cytoplasm, imprisoned host cell mRNA in the nucleus, which suggests 
      a novel mechanism to enhance translation of PRRSV genome. PRRSV nonstructural 
      protein (nsp) 1beta was identified as the nuclear protein playing the role for host 
      mRNA nuclear retention and subversion of host protein synthesis. A SAP (SAF-A/B, 
      Acinus, and PIAS) motif was identified in nsp1beta with the consensus sequence of 
      (126)-LQxxLxxxGL-(135). In situ hybridization unveiled that SAP mutants were 
      unable to cause nuclear retention of host cell mRNAs and did not suppress host 
      protein synthesis. In addition, these SAP mutants reverted PRRSV-nsp1beta-mediated 
      suppression of interferon (IFN) production, IFN signaling, and TNF-alpha production 
      pathway. Using reverse genetics, a series of SAP mutant PRRS viruses, vK124A, 
      vL126A, vG134A, and vL135A were generated. No mRNA nuclear retention was observed 
      during vL126A and vL135A infections. Importantly, vL126A and vL135A did not 
      suppress IFN production. For other arteriviruses, mRNA nuclear accumulation was 
      also observed for LDV-nsp1beta and SHFV-nsp1beta. EAV-nsp1 was exceptional and did not 
      block the host mRNA nuclear export.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Han, Mingyuan
AU  - Han M
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA. Electronic address: 
      hanming@umich.edu.
FAU - Ke, Hanzhong
AU  - Ke H
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Zhang, Qingzhan
AU  - Zhang Q
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Yoo, Dongwan
AU  - Yoo D
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA. Electronic address: 
      dyoo@illinois.edu.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Active Transport, Cell Nucleus/*physiology
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Nucleus/genetics
MH  - Chlorocebus aethiops
MH  - HeLa Cells
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Immunity, Innate/immunology
MH  - Interferons/biosynthesis/metabolism
MH  - Mice
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*physiology
MH  - Protein Biosynthesis/*genetics
MH  - RNA, Messenger/*genetics
MH  - Signal Transduction
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - Virus Replication/*physiology
PMC - PMC7111332
OTO - NOTNLM
OT  - Arterivirus
OT  - Interferon suppression
OT  - MRNA nuclear export
OT  - Nsp1
OT  - PRRSV
OT  - Pathogenesis
OT  - SAP motif
EDAT- 2017/02/27 06:00
MHDA- 2017/05/27 06:00
PMCR- 2017/02/20
CRDT- 2017/02/26 06:00
PHST- 2016/11/16 00:00 [received]
PHST- 2017/02/03 00:00 [revised]
PHST- 2017/02/08 00:00 [accepted]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/05/27 06:00 [medline]
PHST- 2017/02/26 06:00 [entrez]
PHST- 2017/02/20 00:00 [pmc-release]
AID - S0042-6822(17)30036-3 [pii]
AID - 10.1016/j.virol.2017.02.004 [doi]
PST - ppublish
SO  - Virology. 2017 May;505:42-55. doi: 10.1016/j.virol.2017.02.004. Epub 2017 Feb 20.