PMID- 28236059
OWN - NLM
STAT- MEDLINE
DCOM- 20170914
LR  - 20190221
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 470
IP  - 5
DP  - 2017 May
TI  - EWSR1 rearrangement is a frequent event in papillary thyroid carcinoma and in 
      carcinoma of the thyroid with Ewing family tumor elements (CEFTE).
PG  - 517-525
LID - 10.1007/s00428-017-2095-1 [doi]
AB  - Carcinomas of the thyroid with Ewing family tumor element (CEFTEs) are small-cell 
      thyroid tumors with epithelial differentiation that disclose p63 expression and 
      EWSR1-FLI1 rearrangement, carry a favorable prognosis and may co-exist with 
      papillary thyroid carcinoma (PTC) foci. Two histogenetic hypotheses have been 
      advanced regarding the origin of CEFTEs: arising in PTCs or in solid cell nests 
      (SCN). A total of 3 CEFTEs, 54 PTCs, and 10 SCNs were reviewed, and fluorescence 
      in situ hybridization (FISH) technique was performed in all cases to search for 
      the presence of EWSR1 rearrangements. The three CEFTEs disclosed the EWSR1-FLI1 
      rearrangement both in the small cell and in the PTC component. Out of the 54 PTC 
      cases, 28 (51.9%) were positive, 20 (37.0%) were negative, and 6 (11.1%) were 
      inconclusive for EWSR1 rearrangement; in two of the positive PTC cases, the 
      EWSR1-FLI1 rearrangement was detected. Classic PTC disclosed more often the EWSR1 
      rearrangement than other PTC variants (p = 0.031). PTCs with EWSR1 rearrangement 
      disclosed a lower percentage of nuclei with EWSR1 polysomy than those without 
      EWSR1 rearrangement (p = 0.001). Out of the 10 SCNs, 7 (70.0%) were negative and 
      3 (30.0%) were inconclusive for the EWSR1 rearrangement. Monosomic nuclei were 
      more frequent (mean of 44.3%) in SCNs than in PTCs (p < 0.001). The presence of 
      the EWSR1-FLI1 rearrangement in PTC component of all studied CEFTEs and the 
      existence of the EWSR1 rearrangement in some PTCs favor the origin of CEFTE from 
      PTC. The high frequency of EWSR1 rearrangements in PTC may represent a new 
      diagnostic marker of these tumors.
FAU - Oliveira, G
AU  - Oliveira G
AD  - Ipatimup Diagnostics, Institute of Molecular Pathology and Immunology of Porto 
      University, R. Julio Amaral de Carvalho, 45, Ipatimup, 4200-135, Porto, Portugal.
AD  - Institute of Biomedical Science Abel Salazar-ICBAS, University of Porto, Porto, 
      Portugal.
FAU - Polonia, A
AU  - Polonia A
AD  - Ipatimup Diagnostics, Institute of Molecular Pathology and Immunology of Porto 
      University, R. Julio Amaral de Carvalho, 45, Ipatimup, 4200-135, Porto, Portugal.
AD  - Medical Faculty, University of Porto, Porto, Portugal.
FAU - Cameselle-Teijeiro, J M
AU  - Cameselle-Teijeiro JM
AD  - Department of Anatomic Pathology, Clinical University Hospital, Servicio Gallego 
      de Salud (SERGAS), University of Santiago de Compostela, Santiago de Compostela, 
      Spain.
FAU - Leitao, D
AU  - Leitao D
AD  - Ipatimup Diagnostics, Institute of Molecular Pathology and Immunology of Porto 
      University, R. Julio Amaral de Carvalho, 45, Ipatimup, 4200-135, Porto, Portugal.
AD  - Medical Faculty, University of Porto, Porto, Portugal.
FAU - Sapia, S
AU  - Sapia S
AD  - Pathology Department, Vinalopo Hospital, Elche, Spain.
AD  - Pathology Department, Orebro University Hospital, Orebro, Sweden.
FAU - Sobrinho-Simoes, M
AU  - Sobrinho-Simoes M
AD  - Ipatimup Diagnostics, Institute of Molecular Pathology and Immunology of Porto 
      University, R. Julio Amaral de Carvalho, 45, Ipatimup, 4200-135, Porto, Portugal.
AD  - Medical Faculty, University of Porto, Porto, Portugal.
AD  - Anatomic Pathology Service, Centro Hospitalar de Sao Joao, Porto, Portugal.
FAU - Eloy, C
AU  - Eloy C
AD  - Ipatimup Diagnostics, Institute of Molecular Pathology and Immunology of Porto 
      University, R. Julio Amaral de Carvalho, 45, Ipatimup, 4200-135, Porto, Portugal. 
      celoy@ipatimup.pt.
AD  - Medical Faculty, University of Porto, Porto, Portugal. celoy@ipatimup.pt.
LA  - eng
PT  - Journal Article
DEP - 20170224
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (EWSR1-FLI1 fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Carcinoma/*genetics/*pathology
MH  - Carcinoma, Papillary
MH  - Child
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Thyroid Cancer, Papillary
MH  - Thyroid Neoplasms/*genetics/*pathology
MH  - Young Adult
OTO - NOTNLM
OT  - EWSR1 rearrangement
OT  - EWSR1-FLI1
OT  - Ewing sarcoma
OT  - Papillary thyroid carcinoma
OT  - Small cell carcinoma
EDAT- 2017/02/27 06:00
MHDA- 2017/09/15 06:00
CRDT- 2017/02/26 06:00
PHST- 2016/09/18 00:00 [received]
PHST- 2017/02/15 00:00 [accepted]
PHST- 2017/01/23 00:00 [revised]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/09/15 06:00 [medline]
PHST- 2017/02/26 06:00 [entrez]
AID - 10.1007/s00428-017-2095-1 [pii]
AID - 10.1007/s00428-017-2095-1 [doi]
PST - ppublish
SO  - Virchows Arch. 2017 May;470(5):517-525. doi: 10.1007/s00428-017-2095-1. Epub 2017 
      Feb 24.