PMID- 28237035
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20180308
IS  - 1873-281X (Electronic)
IS  - 1472-9792 (Linking)
VI  - 103
DP  - 2017 Mar
TI  - MSMEG_5684 down-regulation in Mycobacterium smegmatis affects its permeability, 
      survival under stress and persistence.
PG  - 61-70
LID - S1472-9792(16)30443-7 [pii]
LID - 10.1016/j.tube.2017.01.004 [doi]
AB  - The Mycobacterium tuberculosis (Mtb) genome sequence and annotation details have 
      been available for a long time; however physiological relevance of many ORFs 
      remains poorly described. Mtb is a pathogenic strain; hence, surrogate strains 
      such as Mycobacterium bovis BCG and Mycobacterium smegmatis (Msmeg) have also 
      been studied to gain an understanding of mycobacterial physiology and metabolism. 
      The Mycobacterium smegmatis mc(2) 155 ORF MSMEG_5684 is annotated as a part of 
      serine biosynthetic pathway, however, its physiological significance remains to 
      be established experimentally. To understand the relevance of SerC for Msmeg 
      physiology we developed a recombinant Mycobacterium smegmatis with SerC knockdown 
      (KD) and also complemented it with serC over-expressing construct (KDC). The KD 
      showed reduced growth compared to wild-type (WT) and complemented strain on 
      glycerol as carbon source. The growth of KD was restored after supplementation of 
      serine. The survival studies with WT and KD under oxidative, nitrosative and 
      detergent stresses showed increased susceptibility of KD. The KD also showed 
      increased susceptibility to antimycobacterial agents and poor ability for 
      in vitro persistence. Also, the serC transcript profiling showed increased 
      expression under stress. The complementation studies with Msmeg serC showed 
      growth restoration of E. coli-DeltaserC in minimal medium.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Keshari, Deepa
AU  - Keshari D
AD  - Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, 
      Sector-10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
FAU - Singh, Kumar Sachin
AU  - Singh KS
AD  - Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, 
      Sector-10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
FAU - Sharma, Rishabh
AU  - Sharma R
AD  - Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, 
      Sector-10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
FAU - Yadav, Shailendra
AU  - Yadav S
AD  - Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, 
      Sector-10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India.
FAU - Singh, Sudheer Kumar
AU  - Singh SK
AD  - Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, 
      Sector-10, Jankipuram Extension, Sitapur Road, Lucknow, 226031, India; Academy of 
      Scientific and Innovative Research (AcSIR), New Delhi, India. Electronic address: 
      sudheer_singh@cdri.res.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170119
PL  - Scotland
TA  - Tuberculosis (Edinb)
JT  - Tuberculosis (Edinburgh, Scotland)
JID - 100971555
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Detergents)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Oxidants)
RN  - EC 2.6.1.- (Transaminases)
RN  - EC 2.6.1.52 (phosphoserine aminotransferase)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Detergents/toxicity
MH  - Down-Regulation
MH  - Gene Expression Regulation, Bacterial
MH  - Gene Expression Regulation, Enzymologic
MH  - Genotype
MH  - Microbial Viability
MH  - Mycobacterium smegmatis/drug effects/genetics/*metabolism/pathogenicity
MH  - Nitric Oxide Donors/toxicity
MH  - Oxidants/toxicity
MH  - Oxidative Stress
MH  - Permeability
MH  - Phenotype
MH  - *Stress, Physiological
MH  - Time Factors
MH  - Transaminases/genetics/*metabolism
OTO - NOTNLM
OT  - Knockdown
OT  - Mycobacterium smegmatis
OT  - Permeability
OT  - Persistence
OT  - Phosphoserine aminotransferase
OT  - Stress
EDAT- 2017/02/27 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/02/27 06:00
PHST- 2016/10/18 00:00 [received]
PHST- 2017/01/06 00:00 [revised]
PHST- 2017/01/17 00:00 [accepted]
PHST- 2017/02/27 06:00 [entrez]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - S1472-9792(16)30443-7 [pii]
AID - 10.1016/j.tube.2017.01.004 [doi]
PST - ppublish
SO  - Tuberculosis (Edinb). 2017 Mar;103:61-70. doi: 10.1016/j.tube.2017.01.004. Epub 
      2017 Jan 19.