PMID- 28237106
OWN - NLM
STAT- MEDLINE
DCOM- 20170815
LR  - 20180920
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 588
DP  - 2017
TI  - Methods to Study Chaperone-Mediated Autophagy.
PG  - 283-305
LID - S0076-6879(16)30372-X [pii]
LID - 10.1016/bs.mie.2016.10.009 [doi]
AB  - Chaperone-mediated autophagy (CMA), a selective form of degradation of cytosolic 
      proteins in lysosomes, contributes to maintenance of proteostasis and to the 
      cellular adaptation to stress. CMA substrates are selectively recognized and 
      delivered by a cytosolic chaperone to the lysosomal surface, where, upon 
      unfolding, they are internalized through a membrane translocation complex. 
      Defective or dysfunctional CMA has been associated with human pathologies such as 
      neurodegeneration, cancer, immunodeficiency, or diabetes, increasing the overall 
      interest in methods to monitor this selective autophagic pathway. In this 
      chapter, we review the different experimental approaches used to evaluate CMA 
      activity in different organs from animals or in cell cultures in vitro.
CI  - (c) 2017 Elsevier Inc. All rights reserved.
FAU - Arias, E
AU  - Arias E
AD  - Institute for Aging Studies, Albert Einstein College of Medicine, Bronx, NY, 
      United States. Electronic address: esperanza.arias-perez@einstein.yu.edu.
LA  - eng
GR  - P30 AG038072/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20161118
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Animals
MH  - *Autophagy
MH  - Biochemistry/methods
MH  - Humans
MH  - Lysosomes/*metabolism
MH  - Molecular Chaperones/*metabolism
MH  - Proteolysis
OTO - NOTNLM
OT  - Autophagy
OT  - Chemical modulators
OT  - Fluorescent reporters
OT  - Lysosomes
OT  - Organelle isolation
EDAT- 2017/02/27 06:00
MHDA- 2017/08/16 06:00
CRDT- 2017/02/27 06:00
PHST- 2017/02/27 06:00 [entrez]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/08/16 06:00 [medline]
AID - S0076-6879(16)30372-X [pii]
AID - 10.1016/bs.mie.2016.10.009 [doi]
PST - ppublish
SO  - Methods Enzymol. 2017;588:283-305. doi: 10.1016/bs.mie.2016.10.009. Epub 2016 Nov 
      18.