PMID- 28237591
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20170522
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 100
IP  - 5
DP  - 2017 May
TI  - Methionine, leucine, isoleucine, or threonine effects on mammary cell signaling 
      and pup growth in lactating mice.
PG  - 4038-4050
LID - S0022-0302(17)30165-0 [pii]
LID - 10.3168/jds.2016-11973 [doi]
AB  - Two studies were undertaken to assess the effects of individual essential AA 
      supplementation of a protein-deficient diet on lactational performance in mice 
      using litter growth rates as a response variable. The first study was designed to 
      establish a dietary protein response curve, and the second to determine the 
      effects of Leu, Ile, Met, and Thr supplementation of a protein-deficient diet on 
      lactational performance. In both studies, dams were fed test diets from 
      parturition through d 17 of lactation, when the studies ended. Mammary tissue was 
      collected on d 17 from mice on the second experiment and analyzed for mammalian 
      target of rapamycin (mTOR) pathway signaling. Supplementation with Ile, Leu, or 
      Met independently increased litter weight gain by 11, 9, and 10%, respectively, 
      as compared with the protein-deficient diet. These responses were supported by 
      independent phosphorylation responses for mTOR and eIF4E binding protein 1 
      (4eBP1). Supplementation of Ile, Leu, and Met increased phosphorylation of mTOR 
      by 55, 34, and 47%, respectively, as compared with the protein-deficient diet. 
      Phosphorylation of 4eBP1 increased in response to Ile and Met supplementation by 
      60 and 40%, respectively. Supplementation of Ile and Met increased 
      phosphorylation of Akt/protein kinase B (Akt) by 41 and 59%, respectively. This 
      work demonstrated that milk production responds nonlinearly to protein supply, 
      and milk production and the mTOR pathway responded independently to 
      supplementation of individual AA. The former demonstrates that a linear 
      breakpoint model is an inappropriate description of the responses, and the latter 
      demonstrates that no single factor limits AA for lactation. Incorporation of a 
      multiple-limiting AA concept and nonlinear responses into milk protein response 
      models will help improve milk yield predictions and allow derivation of diets 
      that will increase postabsorptive N efficiency and reduce N excretion by 
      lactating animals.
CI  - Copyright (c) 2017 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Liu, G M
AU  - Liu GM
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - Hanigan, M D
AU  - Hanigan MD
AD  - Department of Dairy Science, Virginia Tech, Blacksburg 24061.
FAU - Lin, X Y
AU  - Lin XY
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - Zhao, K
AU  - Zhao K
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - Jiang, F G
AU  - Jiang FG
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - White, R R
AU  - White RR
AD  - Department of Dairy Science, Virginia Tech, Blacksburg 24061.
FAU - Wang, Y
AU  - Wang Y
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - Hu, Z Y
AU  - Hu ZY
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China.
FAU - Wang, Z H
AU  - Wang ZH
AD  - Ruminant Nutrition and Physiology Laboratory, College of Animal Science and 
      Technology, Shandong Agricultural University, Taian 271018, P. R. China. 
      Electronic address: zhwang@sdau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170223
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Milk Proteins)
RN  - 04Y7590D77 (Isoleucine)
RN  - 2ZD004190S (Threonine)
RN  - AE28F7PNPL (Methionine)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Animals
MH  - Diet
MH  - Isoleucine/*pharmacology
MH  - Lactation
MH  - Leucine/*pharmacology
MH  - Methionine/metabolism
MH  - Mice
MH  - Milk/metabolism
MH  - Milk Proteins/pharmacology
MH  - Threonine/pharmacology
OTO - NOTNLM
OT  - cell signaling
OT  - litter growth rates
OT  - mice
OT  - single amino acid
EDAT- 2017/02/27 06:00
MHDA- 2017/05/23 06:00
CRDT- 2017/02/27 06:00
PHST- 2016/09/07 00:00 [received]
PHST- 2017/01/03 00:00 [accepted]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
PHST- 2017/02/27 06:00 [entrez]
AID - S0022-0302(17)30165-0 [pii]
AID - 10.3168/jds.2016-11973 [doi]
PST - ppublish
SO  - J Dairy Sci. 2017 May;100(5):4038-4050. doi: 10.3168/jds.2016-11973. Epub 2017 
      Feb 23.